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DNAstar軟件包的使用 第三軍醫(yī)大學(xué)微生物學(xué)教研室 朱軍民 Overview ? DNASTAR develops and supports the best sequence analysis software for life scientists in Pharmaceutical, Biotechnology, Academic and Government Organizations worldwide. DNASTAR軟件包 ? EditSeq ? GeneQuest ? MapDraw ? MegAlign ? PrimerSelect ? Protean ? SeqMan EditSeq 1. Edit and import sequences and annotations ? Translate, backtranslate and reverse plement sequences ? Locate ORFs and create annotations ? Cut/paste sequence with inclusive annotations ? EditSeq included with all systems GeneQuest 2. Discover and annotate genes ? Predict coding regions and splice sites ? Locate repeats, patterns, or areas matching known sequence data ? Simulate agarose gel electrophoresis MapDraw 3. Locate restriction sites ? Create six types of linear and circular maps ? View all six reading frames with sites and features MegAlign 4. Align DNA and protein sequences ? Perform pairwise, multiple and dotplot alignments ? Create phylogeic trees ? Generate subalignments ? Customize alignment displays PrimerSelect 5. Design primers or pare your own primers against a sequence template ? Analyze primers for hairpins and dimers ? View the consequences of primer mutations Protean 6. Predict protein structures ? View PAGE simulations ? Analyze physicochemical properties ? Display results graphically SeqMan 7. Assemble sequences ? Trim poor data and remove vector ? Manage and order contigs ? Locate potential SNPs ? Visualize traces and pare two consensus calls 一、 EditSeq ? EditSeq 是能夠迅速、正確地輸入,并且修改 DNA 或蛋白質(zhì)序列工具。每個(gè)EditSeq 文件都可以分為三個(gè)可編輯的部分,上邊的一部分為序列文件,中間的一部分里是評(píng)論,底部是序列的注釋。 Sequence Entry and Export ? EditSeq