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on. ? Good working knowledge of immunology. ? Excited by the challenge of research and experimentation. RELEVANT SKILLS and ACCOMPLISHMENTS Applied Research ? Successfully developed new antibodies for use in breast cancer research amp。 therapy: experimented with antigen preparation and immunization routines, resulting in the desired immune response。 carefully monitored the antisera to ensure presence of desired Bcell population。 tailored screening strategies using ELISA, RIA and immunoblot techniques, to effectively isolate the desired hybridomas. ? Developed, in collaboration with others, a novel assay which identifies the antibodies39。 ability to bind to live, intact tumor cells. ? Delivered periodic presentations of results and works in progress, to staff of Cancer Research Institute. Innovation/Exploration ? Pursued unique opportunities for experimentation, for example: researched and worked out procedures for in vitro immunization of human lymphocytes。 explored and experimented with hybridoma production, using lymph node cells of a cancer patient。 experimented to induce animals39。 immune system to respond to a weak antigen. EMPLOYMENT HISTORY 1994present Lab Technician SCHILLING CANCER RESEARCH INST., Berkeley 199094 Full time student US Santa Barbara 39。93 summer Research Asst. UC Santa Barbara Biology Dept. 1989 Youth Counselor RAINBOW RIVER DAY CARE PROGRAM, Los Angeles 1988 Emergency Med Tech SEALS AMBULANCE, Costa Mesa 1987 Teaching Asst. ALTA VISTA ELEM. SCHOOL, Redondo Beach Lab Skills ?SDSPAGE Electrophoresis ?Radiolabeling of Antibodies ?Tissue Culture ?Affinity Chromatography ?Immunoblot Strip Assay ?Hamster Egg Peration Test ?Electroblotting ?Lyophilization ?Isotyping EDUCATION ., Cell Biology amp。 Physiology UNIVERSITY OF CALIFORNIA, SANTA BARBARA, 1994 Related coursework: Immunology amp。 Lab, Biochemistry, Virology, Microbiology JOHN BRIDGES page 2 Chronological ? More traditional ? Emphasizes experience