基因工程原理ppt課件(已改無錯字)
2022-10-09 18:25:37 本頁面
【正文】 29 ◆ Cohen與 Boyer合作創(chuàng)建重組 DNA分子 ● To some historians, the discipline of DNA technology came into being over pastrami sandwiches at a local delicatessen in Waikiki Beach. The year was 1972. ● It happened that Herbert Boyer was at a scientific conference in Hawaii speaking about the EcoRl restriction enzyme. Stanley Cohen was in the audience. ● After the presentation, Cohen invited Boyer to lunch to explore their possible collaboration on a series of experiments. ● The two researchers sat and considered the experiments that would send DNA technology into a new era. Cohen在重組 DNA技術(shù)中杰出貢獻(xiàn) 30 ◆ Cohen與 Boyer合作創(chuàng)建重組 DNA分子 ● Cohen had been conducting fruitful experiments with plasmids, but he was experiencing difficulty cutting open the plasmids. ● Boyer39。s EcoRI enzyme seemed to be the ideal solution, so Cohen suggested they join forces. ● They would use his plasmids and Boyer39。s enzyme to rebine the plasmid DNA. ■ First they would try to rebine two plasmids to form a single plasmid. ■ If successful, they would attempt to bring DNA from a foreign species into the plasmid to produce a rebinant DNA molecule. Boyer agreed, and the bargain was struck. Cohen在重組 DNA技術(shù)中杰出貢獻(xiàn) 31 Boyer and Cohen 的策略 32 ◆ pSC102,體內(nèi)構(gòu)建的重組體 ● 他們首先檢查了 EcoRⅠ 對質(zhì)粒pSC101和 R65的切割情況 ， 發(fā)現(xiàn)EcoRⅠ 在質(zhì)粒 pSC101上只有一個切點 ， 在質(zhì)粒 R65上有 12個切點 ,這樣就可以用 pSC101 作為重組DNA的載體分子 。 33 他們用 EcoRⅠ 處理過的和沒有處理過的 質(zhì)粒 pSC101和R65DNA分別轉(zhuǎn)化 C600,得到下表的實驗結(jié)果 ∶ 表：環(huán)狀和線狀質(zhì)粒 DNA的轉(zhuǎn)化
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