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the major virulent factor Department of Microbiology, Harbin Medical University Disease: Brucellosis ? Animal ?Abortion ? Human ?Weakness and fatigue ?Enlarged lymph nodes, liver, and spleen ?Undulant fever (risingandfalling fever) ?Occurs in a few patients Department of Microbiology, Harbin Medical University Diagnosis ? Isolation ?Sample: blood, bone marrow ? Serological examination ?Agglutination test ?Complement fixation test ?Coombs test ? Skin test Department of Microbiology, Harbin Medical University Prevention amp。 Therapy ? Pasteurization of milk ? Immunization ?Animals ?Occupational highrisk persons ? Slaughtering of infected animals ? Treatment with tetracycline and penicillin