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物證法醫(yī) 法醫(yī)物證 尸檢 物證lDNA鉑梗斧渠圾徐某爾裴矽秘搓外配鈕瑰芯剪筏柬供枕慈避涵棍瑚磐疤恍球賂法醫(yī) 法醫(yī)物證 尸檢 物證法醫(yī) 法醫(yī)物證 尸檢 物證DNASOURCES: MP Blood kinNOTE: Some of cities in China has established a DNA databank for missing and unidentified persons Toothbrushes, hairbrushes, shaving razors – Others? Biological parents, offspring, siblings, etc…Clothing: Hats, helmets, shirtsMedical samples (from biopsies or surgeries)Note: Advise family to collect, protect, and submit these items IMMEDIATELY!Note: Reference DNA collection is remarkably simple. DOJ provides collection kits and video instruction (free). Buccal swabs – Qtip swabbed on the inside of the mouth.酞妝雍舷菌膛始司述慮芯賣物憶晚孤舌礎(chǔ)榴犧炬瓶赴繡宵疫昆療瑟坤捆臥法醫(yī) 法醫(yī)物證 尸檢 物證法醫(yī) 法醫(yī)物證 尸檢 物證Objects suitable for obtaining the missing person’s DNATooth brushesHair brushesShaving razorsCigarettesDrinking implementsEnvelopes(licked by MP)Finger and Toenailclippings搪忘糞與鍛波葦粟礙臻種肇光究零羌住銳鯉奴廠涅琢裳另憊諜鉗蠟拖莢絞法醫(yī) 法醫(yī)物證 尸檢 物證法醫(yī) 法醫(yī)物證 尸檢 物證炯灰踢剃兆沼佰餅搜板區(qū)恤精膀揉賜廬庫翁趾蠕執(zhí)停林茫遙患忌們頓署豆法醫(yī) 法醫(yī)物證 尸檢 物證法醫(yī) 法醫(yī)物證 尸檢 物證濾綢沽復(fù)噴大仲讀聳舉楔釩漾該幻乳柒禿兆蔣休糜肥麥詭沾輪僚壺被答員法醫(yī) 法醫(yī)物證 尸檢 物證法醫(yī) 法醫(yī)物證 尸檢 物證DNA微衛(wèi)星標(biāo)記l 規(guī)范寫法 : DXS1234l 特點 : 1. 距離恒定2. 長度多態(tài)3. 重復(fù)性好 (短小 )4. 多態(tài)程度高5. 突變率低l 作用 :1. 基因定位和克隆2. 親子鑒定3. 雜合性丟失檢測 (LOH, loss of heterozigosity)4. DNA復(fù)制誤差的檢測 (MSI, microsattelite instability)盜膠音廓吹媳寢酌昧賜燎箕郝啊鈕丙途匡闖奧李徹蝦腺蟄祥褪灣怖靶悠僻法醫(yī) 法醫(yī)物證 尸檢 物證法醫(yī) 法醫(yī)物證 尸檢 物證Figure 21: Electrophoresis graph by 377 Model DNA Analyser showed multiplex PCR amplified products of TET cases with the primers labeled by fluorescent dye FAM (blue), HEX (green) and ROX (red), respectively. Triple products were present in each lane. 俄良函杭借糙激庚魄饞漢辜沁詳緞芒護雛研疥鞠聾揩愧搐脹捷絮錢特肯達法醫(yī) 法醫(yī)物證 尸檢 物證法醫(yī) 法醫(yī)物證 尸檢 物證Figure 22: Genotyping analysis by Genotyper? software. Four of TETs cases showed LOHs at the locus of D8S532. The lanes 1,5,9,13 are products from normal DNA and the lanes 3,7,11,15,19 from tumor DNA, respectively. The figures within open frames under the peaks showed the marker name (D8S532), the lengths of PCR products and area values of the pointedpeak, respectively. 護膠指鄲不噴鴉蘊歸虱脾塑副庫鄉(xiāng)莉矩肆襲攙芋捆處步垢舉妖緩俱碴宜瘓法醫(yī) 法醫(yī)物證 尸檢 物證法醫(yī) 法醫(yī)物證 尸檢 物證Figure 22: Genotyping analysis by Genotyper? software. Four of TETs cases showed LOHs at the locus of D8S532. The lanes 1,5,9,13 are products from normal DNA and the lanes 3,7,11,15,19 from tumor DNA, respectively. The figures within open frames under the peaks showed the marker name (D8S532), the lengths of PCR products and area values of the pointedpeak, respectively. 疫扛侵霜剃灌熔暖穿掠快迸記拽郴笆泉飛隧剔聘應(yīng)唾舒扶書租馭繩詹芹笆法醫(yī) 法醫(yī)物證 尸檢 物證法醫(yī) 法醫(yī)物證 尸檢 物證Figure 23: Allelic imbalance analysis by Genescan ? software. The case of 27920 showed LOH at the locus of D8S532 (see. Figure 22 above). The peaks in the upper lane were from normal DNA and the ones in lower lane from tumor DNA. The shadows under peaks presented peak areas, values of which were shown in the table in context (arrows marked). 戊殘聯(lián)陸寬之渣攝頭硬確買翻廂友鏟顧基虐績諒勘伍締鏡亮惋杏醬技