分子生物學(xué)-05真核轉(zhuǎn)錄-展示頁
2024-10-25 07:35本頁面
【正文】 for 5S RNA genes is internal。 rRNA transcription。Chapter 5 . Transcription amp。 RNA Processing in Eukaryotic cells,1. RNA polymerase: 3 types,RNA polymerase I: in nucleolus。 the most cellular RNA synthesis. Did not inhibited by aamanitin RNA polymerase II: in nucleoplasm , heterogeneous nuclear RNA (hnRNA) transcription (the precursor for mRNA). inhibited by low concentrations of aamanitin. RNA polymerase III: a minor enzyme activity. in nucleoplasm。 initiation occurs a fixed distance (~55 bp) upstream of the promoter.,RNA polymerase III has a internal promoter,RNA polymerase III has a internal promoter,Promoters for RNA polymerase III consist of bipartite sequences: boxA separated from either boxC or boxB. Or they may consist of separated sequences upstream of the startpoint (Oct, PSE, TATA).,Promoter structure of RNA polymerase III,The binding of the assembly factors TFIIIA and TFIIIC, the initiation factor TFIIIB, and RNA polymerase III.,Promoter structure of RNA polymerase III,TFIIIA: a zinc finger. TFIIIB: three subunits. TFIIIC: at least 5 subunits, 500 kD. TFIIIA and TFIIIC are assembly factors, whose role is to assist the binding of TFIIIB at the right location. TFIIIB functions as a positioning factor, responsible for localizing RNA polymerase correctly. Like SL1
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