新生兒促甲狀腺素的時(shí)間分辨熒光免疫分析及其試劑盒研制-展示頁(yè)
2025-07-08 04:21本頁(yè)面
【正文】 gineering Co.,LTD,Guangzhou 510730 China)Abstract: Objective To establish a method of Timeresolved Fluoroimmunoassay(TrFIA) for the quantitative determination of human thyroid stimulating hormone(hTSH) in blood specimens dired on filter paper as an aid in screening newborns for neonatal hypothyroidism,and to prepare it’s test reagent .Methods The method based on solidphase sandwich TrFIA for Neonatal Hypothyropin(NeohTSH),which used two antiTSH monoclonal antibody one was coated on microplates and another was labeled by Europium. Results The assay sensitivity of the method was ’s curve was was no obvious crossreaction to human luteinizing hormone(hLH) , human follicle stimulating(hFSH) and human chorionic gonadotropin(HCG).The intraassay and the interassay measured values of controls were not more than 25% difference from their labeled values, still their intraassay and the interassay coefficients of variation(CV) of low and high controls were % and %, % and %,respectively. The correlation coefficient of self made Kit and DELFIA 174。試劑盒室間質(zhì)評(píng)能力比對(duì)成績(jī)100%。與DELFIA 174。與DELFIA 174。分析內(nèi)或分析間質(zhì)控結(jié)果在靶值177。結(jié)果 自建的Neonatal hTSH ，且在測(cè)量范圍內(nèi)，自制試劑盒劑量反應(yīng)曲線的相關(guān)系數(shù)r 。新生兒促甲狀腺素的時(shí)間分辨熒光免疫分析及其試劑盒研制譚玉華，馮健明，盧順舵，羅建安，李建國(guó)，季濤，李貴情（廣州市豐華生物工程有限公司，廣州 5107
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