乙肝表面抗原的elisa法定量分析方法學驗證畢業(yè)論文-展示頁
2025-06-28 00:52本頁面
【正文】 .....................................................................3 .............................................................................................3 溶液配制...........................................................................4 鋪板方案...........................................................................4 加樣步驟...........................................................................52. 結果....................................................................................................6 標準曲線與線性范..................................................................6 準確度（回收率）的驗證......................................................6 精密度的驗證..........................................................................7 板內精密度的驗證.........................................................7 板間精密度的驗證.........................................................8 檢測限（LOD）計算.............................................................103. 討論...................................................................................................10 乙肝表面抗原定量分析的意義.............................................10 常用定量分析方法.................................................................11 方法概述................................................................................11 方法學驗證內容....................................................................12 結果分析及應用評價.............................................................13參考文獻................................................................................................14綜述........................................................................................................15 致謝........................................................................................................18中文摘要乙肝表面抗原的ELISA法定量分析方法學驗證 中文摘要目的：對福州藍圖生物工程有限公司生產(chǎn)的HBsAg的ELISA法定量分析試劑盒進行方法學驗證，以判斷是否能用于臨床樣本分析。結果：標準曲線R2=，%，不滿足ng/ml級水平的RSD一般應15%的要求，低濃度樣品（）的檢測在準確性和精密度方面都低于高濃度樣品，不適合低濃度樣品的檢測。關鍵詞：乙肝表面抗原 ELISA 定量分析 方法學驗證1英文摘要The reification of the HBsAg ELISA quantitativmethodologyAbstract In EnglishObject: to verify the quantitative methodology of Fuzhou blueprint biological engineering pany to make sure whether it ca be used to the analysis of Clinical Sample. Methods: ELISA quantitative methodology , make an Application of the standard HBsAg kit from the concentration of 8 ng/ml do 2 times diluted six concentration gradient to constitute a standard curv
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