【正文】
y factors of the biodiesel cost. Many investigations indicated that lipid content and the dry matter yield of microalgae could be influenced by environmental conditions. In the present experiment, the cultural conditions for optimizing lipids productivity in Dunaliella Salina, Isochrysis zhanjiangensis and Nannochloropsis oculata were studied, and a technical route of microalga oil production was built.(1) In the present study, the crude lipid of seven species of marine microalgae were extracted by ethyl ether using soxhlet extraction method, under a temperature of approximately 6080℃. The soxhlet extraction method was improved by dipping the microalgae in ethyl ether for some time before leaching. The results show that the improved soxhlet extraction was more efficient, lower cost and more thoroughly extraction. What’s more the pollution caused by ethyl ether was reduced due to a shorter extraction time.(2) In the present experiment, the effect of CO2 on the growth and lipid content three marine microalgae in two kinds of aerobic culture medium, groupⅠaeration of pure air and groupⅡaeration of mixture with and CO2 for experimental were studied. The results indicated that the logarithmic phase of growth of the three marine microalgae was reached quickly, with the special growth higher than 1, but the growth and lipid content in experimental groupⅠwere significantly lower than that in experimental groupⅡ. At the end of logarithmic phase, the maximum densities of the three marine microalgae were 106, 106 and 107 cell/mL, respectively. The densities were more than , and times in the experimental groupⅡas that in the experimen