【正文】 part of the corn inbred and hybrids. All of t he abovementioned electrophoresis procedures were carried out under basic pH condition. Wang et al. first used t he gradient palmary limed eagle electrophoresis to analyses the 1 mol/ L urea ex tract able proteins of corn seeds under acid pH condition and had been highly praised f or t he possible validity of t his method f or corn seed purity testing. An acid lactatePAGE procedure has been developed in our laboratory as a basic method of w heat cultivar electrophoresis identification. T his procedure was preliminarily proved t o be suit able for separating corn albumin and globulin fractions. The aim of t his study was to evaluate the feasibility of this procedure in distinguishing different corn genotypes, its resolving power, stability, reproducibility and t he genetic expression of parent inbred in term s of band patterns in their F1 hybrids, so as t o explore the possibility of its application on corn genetic purity testing and cultivar identification.1 MATERIALS AND METHODSSample preparationA tot al of 141 inbred and 153 hybrids of corn ( Zea mays) were studied. T he former prised of 9 high oil inbred developed from Illinois high oil ( IHO) C80, 14 high oil inbred from Alexon high oil synthetic C23, and 6 sweet corn inbred from a mercial single cross of Rogers Seed Co. T he latter prised of 26 hybrids w it h on female parents but different male parent s, and 16 w it h mon male but different female parents. All seed samples were provided by our own laboratory . A single corn seed w as ground w it h a sing le seed mill or soaked in tap water under 0 ℃ overnig ht , after which t he embryo and endosperm w ere dissected with a razor blade. After naturally dried, the embryo or endosperm was g round separately. T he ground powder w as put into 2. 5mL centrifuge tube, w it h t he addition of equalvolume of ex tract ion solution( 0. 5 mol/ L NaCl, containing 15% sucrose and 0. 05% methyl green) , mixed thoroughly and extracted for 1 h at room temperature, then centrifuged at 4000 r/ min f or 5 min. The supernatants were used f or electrophoresis.Preparation of the working solutionsT he stock solution, tank buffer, separation gel and concentration gel w ere preparedaccording t o the proportion and volume in T able 1.Table 1 Recipes for stock, extraction and buffer solutionsStock solutionsMixed ratio1 .A crylamide 95 g, Bisacrylamide 3. 8 g, distilled water 500mL2. Sodium lactate2. 81mL+lacticacid to pH3. 2,H2O100mL3 ,ferroussulfate(7H2O)8mg,H2O100mL4. Sodium lactate3mL+lactic acid to pH5. 6,H2O100mL5 A crylamide 26 g, bissacrylamide 5. 2 g, H2O 200mL6 A mmonium persulph at e 11. 41 g, H2O 100mL7 Distilled waterSeparation gel14ml2ml2ml8ul2mlConcertration gel1ml2ml80ulTank buffer: Glycine 4 g+ lactic acid to pH 3. 4, H2O 2000mLElectrophoresisT he vertical plate electro phoretic apparatus w as used w it h 11. 0mm10. 0mm0. 75mm gelslab. Electrophoresis w as carried out at 500 V, 30mA for 1. 5 h. The gel was stained with Coomassie Brilliant Blue R250 ( 40mL 0. 14% Coomassie Brilliant Blue R250alcohol solution dissolved in 12. 5% trichloroacetic acid 160 m L) .2 RESULTS AND DISCUSSIONResolving powerThis electrophoretic procedure showed high resolution t o album ins and globulins of corn seeds. In general, the inbreds or hybrids showed 40 bands or so, up t o over 50, among which t he clearest resolving bands might reach 35. Among all these bands, only one which was heavily stained and stable in location seemed t o be mon t o all inbreds and hybrids. It s relativemigration rat e w as 0. 52 ( Fig. 1,arrow 1) . Another t w o bands also existed in most cases, w it h t heir relative migrationrates of 0. 72 and 0. 40 respectively ( Fig. 1,arrow 2 and 3) . For convenient descript ion, the w hole lot of the bands w ere classified into four groups, designated as α， β ，γ and ω inrelation to the abovementioned three mon or nearly mon bands as markers. T he band group w as fast moving with migration rates ranging from 0. 72 to 1. 0,containing 8 ～ 10 bands. All bands in t his group stained lighter w it h unclear boundary line so it w as difficult t o use them as cultivar identification markers. T he band group w as less fast moving , including bands with migration rates from 0. 52 to 0. 72. 7～9bands could be identified, among which 3～4 bands ha