【正文】
(black bars) treated or not treated with 1 nM BR for 3 h were measured using quantitative RTPCR. BR可以活化miR396b的啟動子活性,但 promGRF5:GUS并沒有明顯減少,說明 BR可能是在轉錄后水平抑制 GRF5基因的表達,而且 BR和miR396b存在潛在聯系,但本文中沒有對其研究 ? miR396 limits mycorrhizal colonization but not nodulation ? We next decided to study the potential role of miR396 in root symbioses. ? We analysed mtrmiR396a and mtrmiR396b expression profiles in roots inoculated with the symbiotic bacteria Sinorhizobium meliloti. 2022/6/4 30 2022/6/4 31 miR396a啟動子首先在根瘤導管組織中發(fā)現, miR396b啟動子在維管組織,感染區(qū)( Ⅱ )和氮固定區(qū)( Ⅲ )含量很高 2022/6/4 32 雖然 miR396存在于根瘤中,但 miR396OE和 MIM396對根瘤密度都沒有明顯影響 ? We also analysed the expression of miR396 in response to MycLCOs, the lipochitooligosaccharide signal molecules produced by AM fungi. ? The involvement of miR396 during mycorrhization was then analysed at 5 weeks after colonization with R. irregularis (formerly Glomus intraradices) in miR396OE and MIM396 roots. 2022/6/4 33 2022/6/4 34 miR396 affects fungal colonization during AM symbiosis. 菌根的叢枝量和形態(tài)沒變化,但 AM定植在miR396OE根中的量減少,而在 MIM396中的量增加 Hence, the action of miR396 limits mycorrhizal colonization. ? Here, we showed that, in the model legume M. truncatula, miR396 represses not only MtGRF but also two MtbHLH79 TFencoding genes, and that its deregulation affects root growth and the ability to be colonized by mycorrhizal fungi. 2022/6/4 35 2022/6/4 36 。3, CYCB2。說明miR396過表達能抑制根的生長 2022/6/4 20 Root meristem size is reduced in miR396OE posite plants 2022/6/4 21 兩者之間沒有大的變化,說明miR396對細胞從細胞周期進入分裂周期的開關沒有大的影響 2022/