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Revision C 5/2023 PN 9000PPT09/26/03 ACL 8000, 9000, and 10000? Clot Curves and DDimer Reaction Curves Hemostasis Technical Marketing 1 Revision C 5/2023 PN 9000PPT09/26/03 Objectives ? Review Nephelometry ? Discuss how clot curves are developed ? Review normal clot curves ? Understand why abnormal clot curves occur ? Review abnormal clot curves 2 Revision C 5/2023 PN 9000PPT09/26/03 What is a clot curve? 3 Revision C 5/2023 PN 9000PPT09/26/03 Tilt Tube Method vs. Instrumentation ? Operator adds reagents and incubates ? Visually determines end point or clot Tilt Tube Instrumentation ? Test is automated ? Instrument takes optical readings ? Uses formula or algorithm to convert optical readings to endpoint 4 Revision C 5/2023 PN 9000PPT09/26/03 Nephelometric Optic Detection Nephelometry is the measurement of light scattered by a particulate solution. Photosensor Light scattered to 90 degrees Light scattered LED at 660 nm 5 Revision C 5/2023 PN 9000PPT09/26/03 Algorithm: A formula or process used to convert the raw optical measurements into a clot time ? Defined in the Test Definition Screen ? Smoothes the data to display a visible line ? Checks for clot integrity ? Raw data that does not pass all criteria will be displayed with an error 6 Revision