疫分析技術(shù)之elisa王娟(參考版)
2025-05-19 12:41本頁(yè)面
【正文】 s mouse encephalomyelitis virus Mouse Toxoplasma Human Varicella zoster Human HEV orf2 3KD Human HEV orf2 6KD Human HEV orf3 3KD Human HIV1 gp120 Human Isotyping ? IgA Human Mouse IgE Human Mouse IgG1 Mouse IgG2alpha Mouse IgG2beta Mouse IgG3 Mouse IgM Human Mouse light chain (kappa or gamma) Mouse Kinase/Phosphorylated Protein ? Akt General Akt/PKB (total) General Akt/PKBpS473 General ATF2 (Thr71) General Erk2 General Erk1 (Thr202/Tyr204) General Erk1/Erk2 (Thr202/Tyr204) General Erk2 (Thr202/Tyr204) General GSK3beta General IkappaBalpha pS32 General IkappaBalpha Total General JNK (pTpY183/185) General JNK Total General JNKp (Thr183/Y182) General MAPKAP K2 General p38 (total) General p38 MAPK pT180/pY182 General p53 (total) General p53 pS15 General PKBalpha General PKC General SAPK1 General SAPK1a/JNK2 General SAPK4 General STAT1 pY701 General STAT1 Total General STAT3 (Tyr705) General ZAP70 General 0 4000 8000 12021 16000 20210 Control LPS IP10 KC MCP1 MIG MIP1? GMCSF IFN? IL10 IL12 IL1? IL2 IL4 IL5 IL6 TNF? 圖 1. BALB/c小鼠內(nèi)毒素休克后 3 h肝臟組織中細(xì)胞因子的表達(dá)譜改變 MFI 0 5000 10000 15000 20210 25000 IP10 KC MCP1 MIG MIP1? GMCSF IFN? IL10 IL12 IL1? IL2 IL4 IL5 IL6 TNF? Control LPS 圖 2. BALB/c小鼠內(nèi)毒素休克后 3 h血漿中細(xì)胞因子的表達(dá)譜改變 MFI 1/2 Future Spectral not Spatial Separation Questions? 。 ?Measuring the level of many human or mouse cytokines in a single sample Readytouse BroadRange Kinase kits for measuring kinase activity ?Ser/Thr Kinase, Tyr Kinase Kit ? Kinase/Phosphorylated Protein ? no washing steps ? faster ? higher throughput ? quantitative assay results ? no hazardous gels / radioactivity ? Sensitivity: higher than ECL/colorimetric assays, parable to or better than radioactive assays Advantages pared to Western blot procedures Applications When to use the LiquiChip System? The LiquiChip System is used for a large variety of protein assays: ? Immunoassays ? Enzyme Assays ? Proteinprotein interaction Assays ? Receptor ligand Assays ? Protein nucleic acid assays Assay Types Immunoassays Enzyme assays (kinase, protease) DNAhybridization assays Interaction assays Realize the Power of Suspension Arrays Assay system that offers: ? multiplexing of assays up to a factor of 100 ? a mixandmeasure procedures (no washing) ? suspension enzyme assays (kinase, phosphatase and protease assays) A utoimmune ASCA Human beta2 Microglobulin Human Mouse Centromere B Human Chromatin Human ENA Profile 4 (SSA, SSB, Sm, RNP) Human ENA Profile 5 (SSA, SSB, Sm, RNP, Scl70) Human ENA Profile 6 (SSA, SSB, Sm, RNP, Scl70, Jo1) Human Histone Human Histone H1 Human Histone H2A Human Histone H2B Human Histone H3 Human Histone H4 Human HSP27 pS82 General HSP27 Total General HSP32 Human HSP65 Human HSP71 Human HSP90 a Human HSP90 b Human Jo1 Human PCNA Human Mouse PR3 Human PR3 (cANCA) Mouse Ribosomal P Human Mouse RNP Human Mouse RNPA Human RNPC Human SCF Human Mouse Scl70 Human Mouse Serum Amyloid P Human SLE Profile 8 (SSA, SSB, Sm, RNP, Scl70, Jo1, RibosomeP, chromatin) Human Sm General Human Smith Mouse Human SSA Human Mouse SSB Human Mouse Streptolysin O Human TPO Human Mouse Allergy Testing Alternaria (Mold) Human Bermuda Grass Human Cat Dander Human Egg White Human Milk Human Mite Pternoyssinus Human Mountain Cedar Human Short Ragweed Human Timothy Grass Human Wheat (food) Human Cancer Markers ? al
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