dns-氨基酸的雙向聚酰胺薄膜層析(參考版)
2025-05-14 12:51本頁面
【正文】 this smear contains the same two pounds which are nicely resolved on the plate next to it. The plate to the far right is a UV visualization of the same overloaded plate. The retention factor, or Rf, is defined as the distance traveled by the pound divided by the distance traveled by the solvent. 。 the left plate is run too concentrated and the spots are running together, making it difficult to get a good and accurate Rf reading. Here39。s kind of hard to see the pencil line in the above photos, so here is a closeup of how the plate looks after the line has been drawn. Under the line, mark lightly the name of the samples you will spot on the plate, or mark numbers for time points. Leave enough space between the samples so that they do not run together, about 4 samples on a 5 cm wide plate is advised. Use a pencil and do not press down so hard that you disturb the surface of the plate. A closeup of a plate labeled 1 2 3 is shown to the right. Spot the TLC plate . . . swirl until dissolved add a few drops of solvent . . . dip the microcap into solution the arrow points to the microcap, it is tiny and hard to see make sure it is filled hold it up to the light if necessary touch the filled microcap to TLC plate to spot it make sure you watch to see that all the liquid has drained from the microcap rinse the micr
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