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2024-12-30 03:23 本頁面
【正文】 and lane 5, primary culture tumor cells derived from grade IV glioblastoma.? LRRC4 arrests U251 cells in G0/G1 phase, it is possible that LRRC4 also induces U251 cells apoptosis. We used the analysis of flow cytometry and AO/EB dual staining to detect U251 cell apoptosis. ? An AO/EB cocktail (80 l) containing 1 ml of DMEM was added in the culture plate. Fields of stained cells were selected and focused using fluorescence microscopy (Nikon Eclipse E800。 Nikon, Tokyo, Japan). Viable cells stained only with AO were bright green with intact structure。LRRC4, a Putative Tumor Suppressor Gene, Requires a Functional Leucinerich Repeat Cassette Domain to Inhibit Proliferation of Glioma Cells In Vitro by Modulating theExtracellular Signalregul
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