[醫(yī)學(xué)]實(shí)驗(yàn)動(dòng)物操作-wenkub.com
2025-01-13 12:39 本頁(yè)面
【正文】 Definition: Transgenic Animal ? Animal in which a segment of DNA has been physically inserted into the genome. ? The genome of all cells of the anism contains the DNA segment. ? The DNA segment is present in the germ line so it can be transmitted to progeny as a Mendelian trait. 1. Test gene function 2. Provide models of specific diseases (progression of disease, alterations in other pathways related to disease, test therapies) 3. Determine function of genes in embryogenesis and in adult life 4. Drug validation Purposes/Applications Two General Classes: 1. Transgenic (overexpressing) 2. Knockout or targeted gene disruption Techniques: 1. Pronuclear transfers: Addition of new geic material Microinjection of DNA into embryo 2. Homologous rebination between exogenous and genomic DNA (the specific replacement of a subset of geic material with other geic material) 3. Inducible/Conditional Knockout Transgenesis in Mice Retroviral Vector Transgenesis in Mice Microinjection Transgenesis in Mice Stem Cell Method ?10%90% egg survival after microinjection ?2060% of eggs surviving microinjection will develop to term ?520% of animals born will carry the transgene A Few Facts about Transgenic Mouse Production Advantages: 1. Can use heterologous promoters to drive tissue or cellspecific gene expression 2. Can produce animals that express the transgene at several different levels 3. Can cross species boundaries。腹腔注射需較高劑量且可能使動(dòng)物死亡時(shí)間延長(zhǎng)及死前掙扎?？蒲兴锜o(wú)法使用麻醉藥時(shí)，使用物理性安樂(lè)死方法。 1000= m2 1608 247。 1000 K為常數(shù)，隨動(dòng)物不同而不同： 小鼠和大鼠 豚鼠 家兔 狗 猴 人 ? 某利尿藥大鼠灌胃給藥時(shí)的劑量為 250mg/kg,試粗略估計(jì)狗灌胃給藥可以試用的劑量。 家 兔 給 藥 灌胃給藥 使用開(kāi)口器和導(dǎo)尿管灌胃。
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