【正文】 ivided by the potential glucose), after different pretreatments. The enzymic hydrolysis yield depended on the temperature of pretreatment. Enzymic hydrolysis yield increased as the temperature was raised. Lower hydrolysis yields were obtained at 190 8C and 4 and 8 min pretreatment conditions, but there were no significant differences with particle sizes.圖3顯示了酶水解產(chǎn)生的水不溶性纖維(表示為一個百分比的葡萄糖產(chǎn)生水解除以潛在葡萄糖),在不同的預(yù)處理。酶水解產(chǎn)生依賴于溫度的預(yù)處理。酶水解收率增加溫度提高。降低水解收率在190 8 c和4和8分鐘預(yù)處理條件,但沒有顯著差異粒徑。Fig3Almost 100% of the theoretical enzymic hydrolysis yield was obtained at 210 8C, for both residence time studied and particle size of 2
5 and 5
8 mm. Lower enzymic saccharification (85%) was obtained at 210 8C, 4 min and a particle size between 8 and 12 optimum pretreatment conditions for the maximum recovery of hemicellulose and cellulose, and for the maximumcellulose enzymatic digestibility are not all the same (Figs. 1
3). In general, pentosans in the liquidfraction and cellulose in the water insoluble fraction require mild severity treatments to be recovered in high yield, but, these are obtained at the expense of decreasing enzymic hydrolysis yield. This fact was not so evident when larger particle sizes (8
12 mm) were used at the highest temperature tested (210 8C). At this temperature cellulose recoveries in waterinsoluble fraction and enzymic hydrolysis close to 90% were obtained.幾乎100%的酶水解得到的理論產(chǎn)量在210 8 c,對于停留時間研究和顆粒大小25和58毫米。降低酶糖化(85%)是在210年獲得8 c,4分鐘和顆粒大小的8至12毫米的最佳預(yù)處理條件的半纖維素和纖維素最大復(fù)蘇,maximumcellulose酶消化率并不都是一樣的(無花果。13)。一般來說,聚糖在液體中分?jǐn)?shù)和纖維素在水中不溶性分?jǐn)?shù)需要溫和的嚴(yán)重程度,治療恢復(fù)正常高收益,但是,這些都是獲得以犧牲減少酶水解屈服。這個事實(shí)并不如此明顯當(dāng)大粒徑(812毫米)都是用在最高溫度測試(210 8 c)。在這個溫度在水不溶性纖維素的復(fù)蘇分?jǐn)?shù)和酶水解得到接近90%。To facilitate the parison of pretreatment conditions focused on the maximum recovery of cellulose on the maximum cellulose accessibility to enzymic attack,Fig. 4shows cellulose to glucose process yield(%) for different pretreatment conditions. This has been calculated as glucose recovery in the water insoluble fibre multiplied by enzymic hydrolysis yield, and divided by potential glucose in raw material. As can be seen process yields about 80% were obtained at 210 8C and 8
12 mm particle size, and these conditions have been considered as the most suitable to pretreatB. carinata straw for the conditions assayed. Thus the utilisation of larger chips in steam explosion of herbaceous agricultural wastes would be desirable to optimise the effectiveness of the process and improve economy by reducing milling power.為了便于比較,預(yù)處理條件集中在最大復(fù)蘇的最大纖維素纖維素的可訪問性,酶的攻擊,無花果。4顯示了纖維素葡萄糖制程良率(%)對不同預(yù)處理條件。這被計算為葡萄糖水不溶性纖維的復(fù)蘇乘以酶水解率,和分裂潛在的葡萄糖在原料?？梢钥吹竭^程收益率約80%在210年獲得了8 c和812毫米的顆粒大小,這些條件被認(rèn)為是最適合pretreatB。carinata稻草的條件化驗。因此,利用更大的芯片在蒸汽爆炸的草本農(nóng)業(yè)廢物將會是可行的優(yōu)化過程的有效性,提高經(jīng)濟(jì)通過減少銑削功率。From the results obtained with B. carinata in this study, we can draw the following conclusions:1) Higher cellulose recoveries at larger particle size (8
12 mm) for all pretreatment conditions tested have been obtained.2) Enzymic hydrolysis yields of 70% were obtained at lower temperature (190 8C) and residence times of 4 and 8 min. Higher enzymic hydrolysis yields (about 99%) were obtained at 210 8C temperature.3) No significant differences in the hemicellulosicsugars recovery were obtained under different pretreatment conditions.4) The best operational conditions were 210 8C, 4
8min and larger particle size, since the highest cellulose recovery and enzymic hydrolysis yields were obtained under such conditions.5) The utilization ofvery small particle size in steamexplosion would not be desirable to optimize the effectiveness of the process and to improve the economy.從得到的結(jié)果carinata b。在本研究中,我們可以得出以下結(jié)論:1)高纖維素的復(fù)蘇在大顆粒大小(812毫米)的預(yù)處理條件測試得到。2)酶水解獲得70%的收益率較低的溫度(190 8 c)和住所的時候4和8分鐘。更高的酶水解收益率(約99%)在210年獲得了8 c溫度。3)無顯著差異hemicellulosicsugars復(fù)蘇,獲得了在不同預(yù)處理條件。4)最好的操作條件是210 8 c,48分鐘和較大的粒子大小、自恢復(fù)和酶水解纖維素最高收率在這樣的情況下。5)利用ofvery小粒徑在steamexplosion不會需要優(yōu)化的過程的有效性和改善經(jīng)濟(jì)。