【導讀】明天擔憂，只要肯做就會有收穫，……在這裡先要特別感謝我的指導老師楊春茂教授，………提供寶貴的意見使我的論文更趨於完善，對未來的研究方向有更多的想法。我也要謝謝實驗室裡由古至今一起打拼的同志兄弟們：…放心自在，謝謝你們，我會永遠愛你們的。狀態(tài)下的調節(jié)及結構重組中扮演重要的角色。當呼吸道發(fā)炎時，氣管會表現(xiàn)cytosolic. 再者，細胞激素及趨化激素已被證實可以調控cPLA2及VCAM-1的表?，F(xiàn)，因而調節(jié)呼吸道疾病的病程。研究調查指出，過敏原、毒害環(huán)境、病毒或細菌感染可以。造成白血球細胞及淋巴細胞浸潤的現(xiàn)象進而導致細胞傷害或是支氣管的發(fā)炎反應。表現(xiàn)，然而對於LPS刺激氣管平滑肌細胞表現(xiàn)cPLA2及VCAM-1的分子機轉仍然並未被證實。我們的假設為LPS經由增加cPLA2及VCAM-1的表現(xiàn)量而影響氣管平滑肌細胞與白。血球細胞之間的相互作用，進而促使呼吸道發(fā)炎反應的產生。氣管平滑肌細胞引發(fā)cPLA2及VCAM-1表現(xiàn)的機轉當中。的方式刺激cPLA2的表現(xiàn)以及PGE2的釋放，此種現(xiàn)象可以受到前處理genistein(tyrosine

　　

【正文】 d SECTION 2: Results SECTION 3: Discussion SECTION 4: Figures and legends This part of thesis had been published on Toxicology and Applied Pharmacology, 2020, 228: 256268 CHAPTER V (Results) Lipopolysaccharide upregulates VCAM1 expression in human tracheal smooth muscle cells via PKC/MAPKs/AP1 activation SECTION 1: Background SECTION 2: Results SECTION 3: Figures and legends SECTION 4: Discussion CHAPTER VI (Results) F ig 1 0 .PK CH TS M CsLPSP4 2 /p 4 4 M AP Kp 3 8 M AP KJ NKc Fo s c J u nV C AM 1c F o s c Ju nAP 1Fig. 10. LPS stimulates VCAM1 expression through PKCs/MAPKs/AP1 pathway. SECTION 1: Conclusion SECTION 2: Perspectives SECTION 3: Schemes CONCLUSION AND PERSPECTIVES CHAPTER VII SECTION 3: Schemes EGF RG ra m n eg a tive ba cte riaLBPLP SC D 1 4TL R4MD 2PI PLC/P C PL CCa2+PK Cs JNKp38 MAP Kp42/p44 MAP KPI3 KNF ? BNucleusNF?BcP LA2M e m bra neW ei n ing 08 ’S c h e m e 1 .S c h e m e 2 .AKTSrcLBPLP SC D 1 4TL R4VCAM1NucleusM e m bra neTFTFp300PYK2EGF RAKTVCAM 1G ra m n eg a tive ba cte riaMD 2PK CsJNKNF ? BNF ? BcFoscJunp38 MAP Kp42/p44 MAP KPI3 KVCAM 1 m RNA a nd pro te in exp r ess io nW ei n ing 08 ’c Fosc Jun1. Wang, ., . Lee, . Lin, . Lin, . Luo, . Wang, and . Yang. Involvement of p42/p44 MAPK, p38 MAPK, JNK and NF?B in interleukin1?induced VCAM1 expression in human tracheal smooth muscle cells. Am. J. Physiol. 288: L227L237, 2020. 2. Luo, ., . Lin, . Yang, . Lee, . Liao, . Leu, and . Hsiao. Induction of cytosolic phospholipase A2 by lipopolysaccharide in canine tracheal smooth muscle cells: involvement of MAPKs and NF?B pathways. Cell. Signal. 18: 12011211, 2020. 3. Lee, ., . Lin, . Lin, . Luo, . Wang, J. Pouyssegur, and . Yang. Transcriptional regulation of VCAM1 expression by tumor necrosis factor? in human tracheal smooth muscle cells: Involvement of MAPKs, NF?B, p300, and histone acetylation. J. Cell. Physiol. 207: 174186, 2020. 4. Liang, ., . Lee, . Lin, . Lin, . Wu, . Luo, and . Yang. Interleukin1β induces MMP9 expression via p42/p44 MAPK, p38 MAPK, JNK and nuclear factor?B signaling pathways in human tracheal smooth muscle cells. J. Cell Physiol. 211:759770, 2020. 5. Lee, ., . Lin, . Lin, . Liang, . Luo, . Wu, . Wang, and . Yang. TNF? induces MMP9 expression via activation of Src/EGFR, PDGFR/PI3K/Akt cascade and promotion of NF?B/p300 binding in human tracheal smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 292: L799L812, 2020. 6. Lin, ., . Lee, . Wang, . Luo, . Wang, and . Yang. Involvement of p42/p44 MAPK, p38 MAPK, JNK and NF?B in LPSinduced VCAM1 expression in human tracheal smooth muscle cells. Cell Signal. 19:12581267, 2020. 7. Lin, ., . Luo, . Wu, Lin ., and Yang . Lipopolysaccharide induces VCAM1 expression and neutrophil adhesion to human tracheal smooth muscle cells: Involvement of Src/EGFR/PI3K/Akt pathway. Toxicol. Appl. Pharmacol. 228:256268, 2020. 8. Luo, ., . Lin, . Chen, . Lin, . Lee, . Lee, . Hsiao, and . Yang. Involvement of MAPKs, NF?B and p300 coactivator in IL1?induced cytosolic phospholipase A2 expression in canine tracheal smooth muscle cells. Toxicol. Appl. Pharmacol. In press, 2020. 9. Lin, ., . Lin, . Luo, and . Yang. Lipopolysaccharide upregulates VCAM1 expression in human tracheal smooth muscle cells via PKC/MAPKs/AP1 activation. In preparation. PUBLICATIONS