【正文】 隨著DNA濃度的增加其峰值不斷變大，且出峰時的波長也變小。對于釕金屬配合物來說，當(dāng)與DNA發(fā)生嵌插作用時，熒光碎滅受到抑制，配合物的熒光強度增強。4 結(jié)論與討論本論文使用水熱法以Ru(III)為金屬離子，2,5–二巰基–1,3,4–噻二唑以及鄰菲啰啉(phen)為配體，合成Ru(III)配合物，得到了晶體樣品。并通過紫外光譜法、熒光光譜法等研究了鄰菲啰啉類金屬配合物與DNA之間的相互作用，通過實驗，可以得出如下結(jié)論：1．水熱合成法可以成功地被使用在此體系的晶體樣品合成中，合成配合物時可重復(fù)性好，為研究配體的配位特征和配合物結(jié)構(gòu)奠定了基礎(chǔ)。2． 配合物為單核離子型化合物。3．通過測定金屬配合物以及與DNA作用的紫外數(shù)據(jù)，可以看出釕金屬配合物的吸收光譜出現(xiàn)增色效應(yīng)和紅移現(xiàn)象，且作用越強增色效應(yīng)越明顯，說明釕金屬配合物與DNA發(fā)生嵌插作用。4．通過熒光研究，說明釕金屬配合物與DNA發(fā)生了嵌插作用。參考文獻：[1] G. F. Swiegers, T. J. Malefetse, New SelfAssembled Structural Motifs in Coordination Chemistry. Chem. Rev., 2000, 100(9): 34833538.[2] B. Rather, M. J. A. Zaworotko, 3D metaorganic network,[Cu(glutarate)2(4,439。bipyridine)],that exhibits singlecrystal to singlecrystal dehydration and rehydration. Chem. Commun., 2003, 7: 830831.[3] Bj?rk N, Cassel A. Metal halide and pseudohalide plexes in dimethyl sulfoxide solution.Ⅱthe crystal structure of bis(dimethylsulfoxide silver (I)perehlorate. Acta Chem. Scandi. Ser. , 30:235240.[4] B F Hoskins, Robson R. Infinite polymeric frameworks consisting of three dimensionally linked rodlike segments. J. Am. Chem. Soc.,1989,111:59625964.[5] Chem C T, Suslick K coordination Polymers: Applications to material science, Coord. Chem. Rev., 1993, 128:193322.[6] J. M. Lehn, Supramolecular Chemistry: Concepts and Perspectives, VCH: Weiinheim, 1995.[7] A. Escherunoser, Rorbert rohsonl Icture, “Post–B12 Prohlems in Corrin synthesis”, Chem. Soc Rev. 1976, 5, 377.[8] M. Devereux, M. McCann, V. Leon, M. Geraghty, V. McKee, J. Wikaira. Synthesis and fungitoxic activity of manganese(II) plexes of fumaric acid: Xray crystal structures of [Mn(fum)(bipy)(H2O)] and [Mn(Phen)2(H2O)2](fum)4H2O (fumH2=fumaric acid。 bipy=2,2′bipyridine。 phen=1,10phenanthroline) polyhedron. 2000, 19:1205～1211.[9] X. M. Zhang, H. S. Wu, X. M. Chen. Linear and helical chains in hydrothermally synthesized coordination polymers [Co(bpdc)(H2O)2] and [Ni(bpdc)(H2O)3]H2O involving in situ ligand synthesis. Eur. J. Inorg. Chem., 2003, 16:2959～2964.[10] N. G. Pschirer, D. , M. D. Smith, W. H. F. Bunz, H. C. zurloye. Noninterpenetrating SquareGrid coordination polymers with dimensions of 2525 197。2 prepared by using N,N39。–type ligands: The first chiral squaregrid coordination polymer. Angew. Chem. Int. Ed. 2002, 41:583～585.[11] G. F. Liu, B. H. Ye. Y. H. Ling, X. M. Chen. Interlocking of molecular rhombi into a 2D polyrotaxane network via p–p interactions. Crystal structure of [Cu2(bpa)2 (phen)2 (H2O)]22H2O. Chem. Commun. 2002,1442～1443.[12] 郭勝利, 殷元琪. 含2,5二巰基1,3,4噻二唑的過渡金屬配合物的合成及性質(zhì)研究. 化學(xué)通報, 1998, 3, 48～50.[13] M. V. Castano, M. M. Plasencia, A. Macias, J. S. Casas, J. Sordo，E. E. Castellano. Preparation and crystal structure of a binuclear pound of methylmercury (II) containing bismuthiol I as a bridging ligand. J. Chem. Soc., Dalton Trans, 1989, 25:1409～1411. [14] P. Mura, B. G. Olby, S. D. Robinson. 1,3,4thiadiazole2,5dithiol plexes of the platinum groupmetals。themolecularstructure of carbonylbis(1,3,4thiadiazole2,5dithiolato)bis (triphenyl phosphine) ruthenium(II). Inorg. Chem. Acta., 1985, 31: 45～52.[15] 呂軍文，曹順安，樓臺芳等. 2,5二巰基1,3,4噻二唑合成方法改進. 化學(xué)工程師, 2003, 5, 15～16.Synthesis、Crystal Structures and Qualitative Research of Ru(III) Complexes with PhenanthrolineMingwu Liu( Department of Chemistry, Dezhou University, Dezhou, Shandong 253023)Abstract：This paper take 2,5dimercapto1,3,4thiadiazole and phenanthroline as a ligand，take the ruthenium as the central ion, obtains this metal preparation crystal using the water heat bonding established law, and using Xray diffraction analysis research model pound crystal structure, using methods and so on fluorescence spectrograph, ultraviolet spectrograph, carries on the structure attribute to the preparation. The experimental result indicated that the chela syngamy body introduction has the decisive influence to the preparation position and the structure and the nature. Through research pound and the DNA and so on between the spectrographic methods had determined the model pound and the DNA function way. Keywords: 2,5Dimercapto1,3,4thiadiazole。 Phenanthroline。 Metal Complexs。 DNA。致 謝首先，我要感謝我的指導(dǎo)老師張秀玲教授對我的關(guān)心和指導(dǎo)。本論文自始至終都是在張老師的悉心指導(dǎo)下完成的，張老師嚴(yán)謹(jǐn)?shù)闹螌W(xué)態(tài)度和勤奮的工作精神使我受益匪淺，不僅使我掌握了很多專業(yè)知識和實驗技能，更重要的是把我?guī)нM了科學(xué)的殿堂，使我對化學(xué)實驗有了全新的、進一步的認(rèn)識，從而為我大學(xué)生涯畫上了一個圓滿的句號。張老師對學(xué)生孜孜不倦的教誨與激勵，學(xué)生將銘記在心。至此論文完成之際，謹(jǐn)向張老師表示誠摯的敬意和衷心的感謝！并祝她身體健康，工作順利！在實驗的過程中，郭鋒老師、劉雷芳老師和朱寶勇老師給予了我無私的指導(dǎo)和熱情的幫助，在此，我向各位老師表示誠摯的謝意。其次，要感謝同實驗組的隋國奇和呂允賀，還有方慶鑫和李煥軍，實驗的順利完成離不開他們的協(xié)作和幫助，同時感謝和我一起跟張老師做論文的其他同學(xué)，大家一起度過快樂寶貴的美好時光。同時我要感謝我的家人，學(xué)業(yè)的順利完成，離不開家人的鼎力支持，父母總是不斷地給予我物質(zhì)支持和精神鼓勵，是他們讓我在學(xué)習(xí)的道路上沒有后顧之憂，不斷向前。最后感謝所有曾給予我支持，鼓勵和關(guān)心的老師，親人和朋友們！