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【正文】 反應(yīng)速率 △ A/min . 通過最小二乘法（ least squares method） 34 Rate A assay – Modular P Reag. at R3 timing Last mp 1st. mp Sample Reag. at R1 timing Rate assays 35 GOT/AST GGT GPT/ ALT Lipase ALP GLDH Amylase LDH CHE CKMB 速率 A 法 分析項(xiàng)目舉例 Enzymes Rate assays 36 2點(diǎn)速率法 2點(diǎn)速率法 尿素氮 指在時(shí)間 吸光度曲線上選擇兩個(gè)測光點(diǎn)，此兩點(diǎn)既非反應(yīng)初始吸光度亦 非終點(diǎn)吸光度，這兩點(diǎn)的單位時(shí)間吸光度差值用于結(jié)果計(jì)算。 37 2point Rate assay Rate assays C + D (product) + B1 (reagent 1) + B2 (reagent 2) A (sample) Enzyme 38 采用一種或多種試劑 檢測兩個(gè)測量點(diǎn)檢測固定的間隔時(shí)間內(nèi)兩點(diǎn)的吸光度變化。 換算成 ? Abs/min參與計(jì)算 在加入最后最后一個(gè)試劑之后進(jìn)行吸光度檢測。 兩點(diǎn)速率法的特點(diǎn) 2point Rate assay Rate assays 39 2point Rate assay – Modular P Rate assays 40 Ammonia Acid phosphatase Nonprostatic phosphatase Urinary/CSF protein Bicarbonate Urea Rate assays 2點(diǎn)速率 法 分析項(xiàng)目舉例 41 Reaction Direction 上升 decreasing 或 下降 increasing 生化反應(yīng)曲線 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 Thank you for your attention Roche Diagnostics (Shanghai) Limited Shanghai 202231 China This presentation is our intellectual property. Without our written consent, it shall neither be copied in any manner, nor used for manufacturing, nor municated to third parties. COBAS and LIFE NEEDS ANSWERS are trademarks of Roche
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