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ering Length of biological relevant elements [1012 cm] [F. Sears (1986), H. Gl228。ttli und M. Goldmann (1987)] XRay Speciality: Anomalous Scattering (ASAXS) Neutrons XRays Intensity low high Hsensitivity high none Isotopesensitivity strong none Heavy elements low high Spinsensitivity strong average Peration depth high low Sample size/amount large small Measurement time long short Interaction with nuclei electron shell electron shell unsystematic a Z Radiation damage none high To Remember: To Remember: H. Zabel, Bochum Contrast Variation When the monster came, Lola, like the peppered moth and the arctic hare, remained motionless and undetected. Harold of course, was immediately devoured. The Concept of Contrast Variation Contrast = Difference of Scattering Length Densities XRay Scattering Neutron Scattering Volume Fraction D2O Scattering Length Density of the Solvent [1010 cm/cm3] Scattering Length Density of the Solute [1010 cm2 ] Water Sugar Contrast = Difference of Scattering Length Densities p(R) = rParticle(R) rLM(R) p(R) = rParticle(R) rLM r(R) = Scattering Length Densitiy = Sum of Scattering Length of all Atoms in a Volume The Concept of Contrast Variation Contrast = Difference of Scattering Length Densities ? Change of solvent position ? Change of sample position Isotopic substitution ? Change of scat