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The TATA box tends to be surrounded by G Crich sequences. It is almost identical with the 10 sequence found in bacterial promoters. The minority of promoters that do not contain a TATA element are called TATAless promoters. TBP: TATAbinding protein, ~30 kD. TAFs: for TBPassociated factors. The assembles at the promoter Promoters for RNA polymerase II have short sequence elements CAAT box : 75, CAAT. a mon elements, functions in either orientation. To increases promoter strength. GC box: 90, GGGCGG. Often multiple copies. in either orientation. a relatively mon promoter ponent. Promoters for RNA polymerase II have short sequence elements Promoters for RNA polymerase II have short sequence elements 3. Enhancer ? bidirectional elements that assist initiation ? function in either orientation and in any location (upstream or downstream) relative to the promoter An enhancer in the virus SV40 It contains two identical sequences of 72 bp each, repeated in tandem ~200 bp upstream of the startpoint of a transcription unit. Each 72 bp repeat contains a copy of the enhancer 4. RNA processing ? mRNA processing ? 5’ Capping ? 3