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Exploitation of phosphorescent labelling reagent of fullerolfluorescein isothiocyanate and new method for the determination of trace alkaline phosphatase as well as forecast of human Chimica Acta, 2020, in prees 原始數(shù)據(jù) 磷光光譜 3 0 0 . 0 350 400 450 500 550 600 650 700 750 7 8 0 . 01 1 . 74060801001201401601802002202 4 0 . 0nm 1 139。2 239。3 339。4439。5 539。6 639。7 739。4 1 2 . 2 0 , 4 8 . 0 85 8 4 . 9 9 , 4 5 . 0 36 2 3 . 0 2 , 2 1 3 .7 14 5 7 . 0 4 , 2 1 2 .3 54 5 7 . 3 8 , 2 1 1 .2 6 6 2 3 . 0 6 , 2 1 1 .7 86 2 1 . 9 4 , 9 4 . 1 24 5 6 . 0 1 , 9 4 . 4 44 6 8 . 5 7 , 5 3 . 2 7 6 3 4 . 3 9 , 5 5 . 0 06 2 1 . 9 5 , 6 6 . 7 94 5 7 . 1 3 , 6 7 . 1 46 4 5 . 2 2 , 7 9 . 4 94 7 8 . 8 5 , 7 9 . 2 4 3 0 0 . 0 350 400 450 500 550 600 650 700 750 7 8 0 . 01 1 . 74060801001201401601802002202 4 0 . 0nm 1139。2239。3339。4439。5539。6639。7739。 圖 1 FolTween80Cyt C 體系 的室溫磷光光譜 λex λem Ip △ Ip △λ ’ NCM ’ ag spot－ 1 Cyt C ’ ’+ ag spot－ 1 Cyt C － ’ ml F–ol ’ ’ + ag spot－ 1Cyt C ’ ’ + ag spot－ 1Cyt C ΔIp λ/nm 14 ’ ’ + ml Tween80 － ’ lsx111。 lsx113 ’ lsx311。 lsx315 ’ lsx336。 lsx334 ’ lsx404。 lsx407 ’lsx276。 lsx275 ’ lsx208。 lsx209 ’ lsx134。 lsx135 ’ NCM ’ ag spot－ 1 Cyt C ’ ’+ ag spot－ 1 Cyt C ’ ml F–ol ’ ’ + ag spot－ 1Cyt C ’ ’ + ag spot－ 1Cyt C ’ ’ + ml Tween80 最佳條件 表 1. 檢測 條件 Reagents Concentrations and volumes ΔIp Optimal Fol (10－ 4 mol L－ 1) (ml) , , , ,, , , , , , , , 10－ 5 mol L－ 1 mL Tween80 (1%) (mL) , , , , , , , , , , , , , , , , , , % mL Pb2+ (10－ 4 mol L－ 1) , , , , , , , , 體系的 pH , , , , , , , , , , , , , , , , 反應溫度 (℃ ) , , , , , , , , , , , 50 ℃ 反應時間 (min) 5, 10, 15, 20, 25 , , , , 15 min 烘干溫度 50, 60, 70, 80, 90, 95 , , , , , 90℃ 烘干時間 , , , , , , , , , , 2 min 放置 時間 10, 20, 30, 40, 50, 60, 70, 80 , , , , , , , 60 min 圖 1 檢測 條件 ΔIp Optimal 固體基質 PAM(a), ACM(b), NCM(c), Paper(d), , , ,