【正文】 ge 1 6. Excise a meristem () from a shoot tip 7. Place it aseptically on sterilized medium Medium with sucrose 30 g L1 meristem ? MS+ +3%蔗糖 ? 溫度 ： 21~25℃ ? 光照度 ： 20223000 lx ? 光照時(shí)間 ： 12h/d 23周長愈傷， 45周長綠點(diǎn)， 36月長成 23厘米小芽，越慢越好，出芽很快的扔掉。 (3)葉 全緣，心臟形。 馬鈴薯生物學(xué)特性 馬鈴薯性喜冷氣候，不耐高溫和霜凍。 馬鈴薯繼代培養(yǎng)基： After culturing for 34 weeks, elongated shoots are transferred to multiplication medium Stage 2 4 weeks 分割移植 3 6 Principle of multiplication Nodal proliferation， Axillary bud formation One nodal cutting one plant，high geics，simple operation, higher survival exvitro，but lower multiplication rate at beginning. 6 3 6 shoots 6 shoots Nodal cuttings CUT CUT Axillary bud formation Restrain topbud growth, enhance formations of axilary buds High geics， simple operation Axillary branching Next explant CUT Principle of multiplication Young shoots or bud grown on a appropriate culture medium bee cluster shoots. Higher multiplication rate with higher mutation when high phytohormone us