【正文】 測序。 令人感興趣的是，魚類中 GtHⅡ基因除了在性腺中有豐富的表達外，在非性腺組織中也有少量或微量表達 [3,16]；但迄今為止還尚未見有對齊口裂腹魚 GtHRⅡ 基因的克隆及序列研究，因此，本試驗通過 對齊口裂腹魚 GtHRⅡ 基因 的克隆及序列分析 ，闡明促性腺激素受體在齊口裂腹魚生殖周期的生理作用 , 以期為闡明齊口裂腹魚繁殖調(diào)控機制提供科學(xué)依據(jù)。 PCR產(chǎn)物回收及克隆測序 用 Omega凝膠回收試劑盒純化回收 PCR產(chǎn)物，將稀釋的 DNA保存在20℃冰箱中備用。 蛋白質(zhì)磷酸化位點分析 圖 9 齊口裂腹魚 GtHRⅡ 蛋白磷酸化位點預(yù)測 Fig. 9 Protein phosphorylation site prediction of Schizothorax prenanti GtHRⅡ 由上 圖可知， GtHRⅡ 蛋白中含有 4 個磷酸化位點，其中 Ser（ Serine 絲氨酸）位點有 4 個，沒有 Thr（ threonine 蘇氨酸）、 Tyr（ tyrosine 酪氨酸）的磷酸化位點。 13 參考文獻 [1] Kraak GVD, Suzuki K, Peter RE, et al. ProPerties of mon carp gonadotroPin I and gonadotroPin Ⅱ [J]. General and ComParative Endocrinology 1992, 85(2): 217229 [2] Oba Y, Hirai T, Yoshiura Y, et al. The duality of fish gonadotropin receptor： Cloning，functional characterization of a second gonaotropin receptor cDNA expressed in the ovary and testis of amago salmon (oncorhynchus rhodurus)[J]. Biochem Biophys Res Commun, 1999, 265(2):366371. [3] Oba Y, Hirai T, Yoshiura Y, et al. Cloning, functional characterization, and expression of a gonadotropin receptor cDNA in the ovary and testis of amago salmon(Oncorhynchus rhodurus)[J].Biochemical and Biophysical Research Communications, 1999,263(2): 584590. [4] Kumar R S, Ijiri S, Trant J M. Molecular biology of the channel catfish gonadotropin receptors:1. Cloning of a functional luteinizing hormone receptor and preovulatory induction of gene expression[J]. Biol Reprod, 2021,64(3):10101018. [5] Kumar R S, Ijiri S, Trant J M. Molecular biology of the channel catfish gonadotropin receptors: 2. Complementary DNA cloning,functional expression,and seasonal gene expression of the folliclestimulating hormone receptor[J]. Biol Reprod, 2021,65(3):710717. [6] Swanson P, Dittman A. Pituitary gonadotropins and their receptors in fish[C] // Advances in Comparative :Bologna,1997. 841856. [7] Vischer H F, Bogerd J. Cloning and functional characterization of a gonadal luteinizing hormone receptor plementary DNA from the African catfish (Clarias gariepinus)[J]. Biol Reprod, 2021, 68(1): 262271. [8] Bojerd J, Blomenrohr M, Andersson E, et al. Discrepancy between molecular structure and ligand selectivity of a testicular folliclestimulating hormone receptor of the African catfish (Clarias garie Pinus)[J]. Biol Reprod, 2021, 64(6): 16331643. [9] Rocha A, G243。 表 1 齊口裂腹魚 GtHRⅡ 基因編碼的氨基酸組成 Table 1 Amino acid of Schizothorax prenanti GtHRⅡ gene encoding Amino Acid Single letter abbreviations Acid Number Mol% Amino