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.................................................... 16 不同種類、濃度的激素配合使用對銀斑百里香 無菌苗生長的影響 .............................................. 3 摘要 ................................................................................................................................................................. 3 引言 ................................................................................................................................................................. 4 1. 試驗材料與方法 ..................................................................................................................................... 4 供試材料 ................................................................................................................................................ 4 培養(yǎng)基配制 ............................................................................................................................................ 4 培養(yǎng)條件 ................................................................................................................................................ 5 接種條件及培養(yǎng)方法 .......................................................................................................................... 5 ................................................................................................................................................ 5 不同濃度的 NAA 與 6BA 配合使用對銀斑百里香的影響 ...................................................... 5 NAA 與 6BA 配合 使用對銀斑百里香種子發(fā)芽率的影響 ..................................................... 5 NAA與 6BA 配比對銀斑百里香無菌苗生長的影響 .............................................................. 6 不同濃度的 2,4D和 6BA 配合使用對銀斑百里香的影響 ...................................................... 6 2,4D和 6BA 配合使用對銀斑百里香種子的影響 ............................................................... 6 2,4D和 6BA 配合使用對百里香無菌苗生長狀況的影響 .................................................. 7 ................................................................................................................................................ 8 ..................................................................................................................................................... 9 附錄: ......................................................................................................................... 錯誤 !未定義書簽。 1. 試驗材料與方法 供 試材料 銀斑百里香 (‘ Silver Posie’ )( 黃健等 2021 )種子 ,于 2021 年 9 月 采自張掖市大滿鄉(xiāng)。 由表 3可知, A2 B2 和 A2 B3處理與其他濃度組合間均達到極顯著水平, 無菌苗生長狀況較 好; A3B A1B4和 A2B1之間無差異顯著性; A4濃度下的各個濃度組合使下的幼苗生長狀況較差 。李曉東等 (2021)分別 以普通百里香的帶腋芽的莖段和種子為外植體, 比較了不同濃度的 IAA 和 6BA 組合對不定芽的誘導(dǎo)和增殖的效果 ; 2021 年又 設(shè)計了不定芽增 殖 培養(yǎng)基 MS+NAA( 0~ ) +6BA(0~ 2mg/L),發(fā)現(xiàn)不定芽的增 殖 受 6BA的影響,當(dāng) NAA 濃度為 ,隨 6BA 濃度的增加,不定芽的增 殖 系數(shù)降低。最后,感謝各位評審老師在百忙之中對我的論 文的批閱與指導(dǎo)!道一聲:“老師您辛苦了”! 此致 敬禮 農(nóng)業(yè)與生物技術(shù)學(xué)院園藝專業(yè) 2021 屆本科生畢業(yè)論文 11 學(xué)生:張亞萍 2021 年 6月 10日 農(nóng)業(yè)與生物技術(shù)學(xué)院園藝專業(yè) 2021 屆本科生畢業(yè)論文 12 河西學(xué)院本科生畢業(yè)論文(設(shè)計)開題報告 論文題目 不同種類、濃度的激素配合使用對銀 斑 百里香無菌苗生長的影響 學(xué)生姓名 所屬學(xué)院 農(nóng)業(yè)與生物技術(shù)學(xué)院 專業(yè) 園藝 年級 2021 級 指導(dǎo)教師 所在單位 河西學(xué)院 職稱 副教授 開題日期 2021 年 3 月 選題的根據(jù):選題的理論、實際意義并綜述有關(guān)本選題的研究動態(tài)和自己的見解 1. 選題的理論、實際意義 百里香 ( Thymus mongolicus), 俗稱 “ 地椒、地花椒 ” ,是唇形科半灌木。 銀斑百里香是河西地區(qū)普遍生長的一種植物 , 屬于百里香屬唇形科 半灌木 。而在培養(yǎng)基中適當(dāng)降低 6BA 濃度,可以使芽伸長。 論文的主要內(nèi)容、基本要求及其主要的研究方法: 試驗材料與方法 試驗材料 銀斑百里香 (‘ Silver Posie’ ) 種子( 于 2021 年 9 月 采自張掖市大滿鄉(xiāng)) 。re looking at rocks that are 2 billion years old. Some of the harsh striations have been dated to the tectonic plate shifts of 65 million years ago that broke up the Earth39。s not have any talk of mutiny on this ship, he jokes. Always remember: You are outnumbered.They are safe, easy and family friendly, no wonder growing numbers of people are spending their vacations on a cruise ship. Zhou Wenting goes aboard. Imagine spending a holiday in a place where your every whim is catered for, where the sun and the ocean are on your doorstep and delicious food is served around the clock. You can go shopping in stores that have rock bottom prices, and if you grow tired of all the pampering, your bed is available anytime. You can travel with the whole family too, because there are activities tailored to suit all age groups. A vacation on an elegant cruise liner will meet the desire for an exotic experience, but will also be an enjoyable travel experience when acpanied by young children or elderly parents. Familyfriendly, safe and secure, cruise vacations have bee very popular in China. During the uping National Day Golden Week holiday, from Oct 1 to Oct 7, many families will board a ship to enjoy an exciting holiday at sea. The development of the cruise industry in recent years has been impressive. Statistics from the China Cruise and Yacht Industry Association show Chinese residents made million trips on cruise liners last year, pared with nearly 10,000 in 2021. The association predicts the market will continue to boom in the next two years. Residents of Shanghai, the city th at boasts the largest cruise ports of the four on the Chinese mainland, are more likely to book cruises than people in other parts of the country, according to a report published by Baidu Inc39。m slightly surprised to find that quite a few passengers have not only cruised with Silversea before, but rather often. At one reception, a Japanese lady gets applauded as the passenger aboard having the most cruise days with the line: 860! That39。s how our shipboard naturalist John Fonseca describes the slushy ice that the Silver Explorer is plowing through as we enter Greenland39。 (1999)以百里香 ( L.)莖段為外植體, MS 為基本培養(yǎng)基,認(rèn)為 , 1mg/LNAA 有利于生根。其最適生根培養(yǎng)基為 1/2M