【正文】 (1):4147. 趙慶臻 ,閻華超 ,程霜等 .地椒的組織培養(yǎng)及植株再生 [J].植物生理學(xué)通訊 , 2021,38(4):357. 朱至清 .植物細(xì)胞工程 [M] .北京 :化學(xué)工業(yè)出版社 , 2021,28 29. 致謝 首先感謝四年來學(xué)院各位老師對我的關(guān)心與指導(dǎo)！為我的人生添磚加瓦。全球百里香屬植物有 400 多個(gè)種與亞種， 100 多個(gè)栽培品種。由于含有芳香精油，還可用于香水、香皂、化妝品等日用工業(yè)品， 在醫(yī)藥、傳統(tǒng)日用化工及精細(xì)化工等領(lǐng)域得到廣泛應(yīng)用。所以，加強(qiáng)百里香在城市園林綠化應(yīng)用方面的研究，對于豐富甘肅乃至全國城市園林綠化樹種選擇具有重要意義。 目前對河西地區(qū)百里香的生境、分布特點(diǎn)、種質(zhì)資源等至今還沒有人進(jìn)行過系統(tǒng)調(diào)查 ， 品種選育更是空白，生產(chǎn)上采用的品種仍為半野生或農(nóng)家品種 。它資源豐富，經(jīng)濟(jì)價(jià)值高，兼有較高的食用、藥用和化工應(yīng)用價(jià)值，同時(shí)還有香化，綠化和環(huán)保等作用，市場前景廣闊。不定芽增殖最適宜的培養(yǎng)基為 MS＋ ＋，增殖系數(shù)高達(dá) 。 魏艷等 (2021)以百里香頂芽和腋芽為試材， MS 為 基本培養(yǎng)基； MS++ mg/L +% 瓊脂 +3%蔗糖為增殖培養(yǎng)； l/2MS+ mg/L +%瓊脂 +2%蔗糖 生根培養(yǎng)基，對河北地區(qū)百里香進(jìn)行了組織培養(yǎng)。 農(nóng)業(yè)與生物技術(shù)學(xué)院園藝專業(yè) 2021 屆本科生畢業(yè)論文 14 李曉東等 (2021)試驗(yàn)建立了一種普通百里 香的快速繁殖方法。如果更換培養(yǎng)基可提高芽增殖效率，不定芽在 MS＋ ＋ ，因而在短時(shí)間內(nèi)就可以獲得大量普通百 里 香（ T． vulgaris）枝條。在愈傷組織誘導(dǎo)中 2,4D 與 NAA 的濃度比在 ~ 之間時(shí)誘導(dǎo)此種百里香的愈傷組織的效 果較好； 2,4D 與 NAA 濃度比大于或等于 2 時(shí)不能誘導(dǎo)出該種百里香的愈傷組織； 2,4D 與 NAA 的濃度比小于 時(shí)也不能誘導(dǎo)出該種百里香的愈傷組織。雖百里香屬植物的組培快繁研究已歷時(shí)多年，但組培技還未得到廣泛應(yīng)用。 農(nóng)業(yè)與生物技術(shù)學(xué)院園藝專業(yè) 2021 屆本科生畢業(yè)論文 15 對本試驗(yàn)的 見解 近十多年來 ， 人們開始采用組織培養(yǎng)的方法獲得銀斑百里香的無菌幼苗或者誘導(dǎo)愈傷組織，篩選出不同培養(yǎng)目的下的優(yōu)良激素組合。 試驗(yàn) 驗(yàn)方法 以 MS 培養(yǎng)基為基本培養(yǎng)基， NAA 設(shè) 、 、 、 濃度， 6BA 設(shè) 、 、 、 ， 2,4D 設(shè) 、 、 、 。 選題要求 農(nóng)業(yè)與生物技術(shù)學(xué)院園藝專業(yè) 2021 屆本科生畢業(yè)論文 16 （ 1）選題后，認(rèn)真查閱相關(guān)文獻(xiàn)，閱讀領(lǐng)會(huì)畢業(yè)論文 的 內(nèi)容和要求，制定試驗(yàn)計(jì)劃、工 作計(jì)劃和進(jìn)度表，做好各種準(zhǔn)備工作。 主要參考資料和文獻(xiàn) ： 曹軍勝 .百里香保健醬油的研制 [J]. 中國釀造 , 2021 ,2930. 段繼功 .紅枸杞百里香保健茶 [J]. 中國土特產(chǎn) , 1994,(6):32. FURMANOWAM， OLSZOWSKAO． Micropropagation of Thyme［ J］． In： BajajYPS（ Ed） Bioteclmology in Agriculture and Forestry, 1992(2)。s Prince Christian Sound, named after a longago Danish royal who became King Christian VIII. In the distance, the water surface is a smooth sheet of darkgreen jade under the overcast sky. The cruise ship suddenly feels larger as we slip into the narrow passage of the sound. Bigger and bigger chunks of sea ice float by, like huge gobs of whipped cream dropped on the surface by some quirky Norse god. The sculpted forms pass so close, it39。s landmass into the continents we know today. There are about 50 passengers on board, most huddled among the rails at 8 am in the bright red parkas provided by the Silversea cruise line. The air temperature is 9 C－ the water temperature is12 C, expedition leader Stefan Kredel announces on a loudspeaker, just in case anybody is planning to strip off for a morning swim. Our luxury vessel is small as cruise ships go. American TV39。re on the wrong day, Silver Explorer works its way as close to shore as possible before droppinganchor. Then we pull on our waterproof pants, jackets and boots, and climb single file intoZodiacs－ the big, motorized rubber rafts that will take us to the day39。re promised a landing near two thermal pools with a view of passing icebergs. Those swimming suits we packed will be used after all. Each day, there is at least one lecture by 農(nóng)業(yè)與生物技術(shù)學(xué)院園藝專業(yè) 2021 屆本科生畢業(yè)論文 18 an expert－ a geologist, birder, Viking scholar, botanist, anthropologist or marine biologist. These informal talks are fortably spaced between gourmet meals and free flowing wine and spirits, all of which can be enjoye d in the collegiality of the restaurant or in the cozy privacy of your cabin. The cabins are surprisingly spacious, and the butler assigned to my suite keeps the plimentary minibar stocked with my preferred beverages and snacks. He and the cabin valet will discreetly tidy up the room each day while I39。s getting close to three years at sea. On this cruise, we are particularly pampered: There are only 56 passengers－ and 119 crewmembers. There are usually 120 to 150 passengers on board, and most cruises are fully booked－ popular destinations such as Antarctica and the Mediterranean as much as a year ahead. Late summer timing and the challenge of booking return flights after disembarking at Kangerlussuaq have kept our group on the small side. We39。s travel website last year. Tourists from Shanghai are also willing to spend more, many paying 4,000 yuan ($652) to 6,000 yuan, to take a cruise. Xue Yifan, who returned last week from a sixday cruise trip to Japan and South Korea with her 6yearold son and parents, paid 8,000 yuan for the trip per person. She says the itinerary arranged for the guests was amazing. Life39。s club from morning to evening and seniors have great fun in the karaoke bar and cinema. I saw some older people in wheelchairs on board. Cruise travel is fabulous for everybody, says Xue, a 35yearold from Shanghai. She says she is keen to try the Royal Caribbean’s newest cruise ship, the 18deck Quantum of the Seas that can carry 4,180 passengers, which is moving to Shanghai in May. Carnival, Royal Caribbean39。s first auto train ready to serve Life39。ll be extrapampered with two crewmembers on board to every one passenger. But let39。s no assigned seating, except at the wele and farewell dinners hosted by Croatian captain Denis Radja) features gourmet European fare. Lunches are expansive buffets in the restaurant－ though in good weather, many of us sit in the sun on the observation deck, where there is a small buffet as well as sandwiches and pastas made to order. I39。s last yellow wildflowers. We explore an outdoor museum, watch a kayak demonstration at a traditional hunting camp, enjoy an Inuit choir performance in the church, and enjoy coffee and a folk dancing at the local culture center. Nanortalik means place of polar bears in the local Inuit language, but a fanged ivory skull in the muse um is the closest we39。s relative pettiness is key to our itinerary. At the moment, we are cruising overa channel 240 meters deep, but yesterday, winding through Skoldungen Fjord, we hadscooted over depths as