【正文】 ................. 7 固定化酶和細胞 ..................................................................................................................... 9 9 國內的需求情況 ..................................................................................................................... 9 .......................................................................................................................... 9 國內幾大生產(chǎn)廠家 ..............................................................................................................10 10 本設計的目的 ........................................................................................................................10 本設計的主要內容 ..............................................................................................................10 2 全廠工藝流程及論 證 12 12 無菌空氣制備系統(tǒng)工段工藝論證 ................................................................................12 13 發(fā)酵工藝流程 ........................................................................................................................13 菌種選取 ..................................................................................................................................13 培養(yǎng)基 .......................................................................................................................................14 生產(chǎn)方法 ..................................................................................................................................14 發(fā)酵過程的控制 ...................................................................................................................14 15 后提取工藝流程 ...................................................................................................................15 提取方法論證 ........................................................................................................................15 3 纖維素酶的工藝計算 18 中國礦業(yè)大學 2020 屆本科生畢業(yè)設計 第 5 頁 18 工藝指標 ..................................................................................................................................18 發(fā)酵工段的物料衡算 .........................................................................................................18 提取工段的物料衡算 .........................................................................................................19 20 蒸氣消耗計算 ........................................................................................................................20 22 種子罐冷卻水 ........................................................................................................................22 發(fā)酵 罐冷卻水 ........................................................................................................................22 22 發(fā)酵罐通風量的計算 .........................................................................................................22 種子培養(yǎng)基等其他無菌空氣耗量 ................................................................................22 發(fā)酵車間高峰無菌空氣消耗量： ................................................................................22 發(fā)酵車間年用氣量： .........................................................................................................22 4 纖維素酶發(fā)酵工段的設備選型與計算 24 24 發(fā)酵罐的選型 ........................................................................................................................24 生產(chǎn)能力、數(shù)量和容積的確定 .....................................................................................24 發(fā)酵罐基本尺寸確定 .........................................................................................................24 冷卻面積的計算 ...................................................................................................................25 蛇管設計 ..................................................................................................................................27 壁厚計算 ..................................................................................................................................29 攪拌器計算 .............................................................................................................................29 ...................................................................................................................30 接管設計 ..................................................................................................................................32 傳動裝置設計 .....................................................................................................................33 發(fā)酵罐支座選擇 ................................................................................................................33 33 種子罐的選 型 ........................................................................................................................33 種子罐容積和數(shù)量確定 ....................................................................................................33 主要尺寸確定 ........................................................................................................................34 冷卻面積的計算 ...................................................................................................................34 設備材料選擇 ........................................................................................................................35 壁厚計算 ..................................................................................................................................35 種子罐內部結構的工藝計算 ..........................................................................................36 支座選型 ..................................................................................................................................38 38 種子罐分過濾器 ...................................................................................................................38 發(fā)酵罐分過濾器 ...................................................................................................................39 40