大鼠(rat)血管內皮生長因子受體2(vegfr-2)-newa(完整版)
【正文】 this VEGFR2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus VEGFR2 concentration. The concentration of VEGFR2 in the samples is then determined by paring the . of the samples to the standard curve.Sample collection and storagesSerum Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000g. Remove serum and assay immediately or aliquot and store samples at 20℃ or 80℃.Avoid repeated freezethaw cyclesPlasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000g at 28℃ within 30 minutes of collection. Store samples at 20℃or 80℃. Avoid repeated freezethaw cycles.Cell culture supernates and other biological fluids Remove particulates by centrifugation and assay immediately or aliquot and store samples at 20℃or 80℃. Avoid repeated freezethaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate
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鄂ICP備17016276號-1