【正文】 ..............................2 大豆耐鹽堿性鑒定 .......................................................................................................2 耐鹽堿的鑒定方法 .................................................................................................2 田間鑒定 ..........................................................................................................2 室內(nèi)鑒定 ..........................................................................................................2 大豆鹽害鑒定指標(biāo) .................................................................................................3 相對鹽害指數(shù)法 ..............................................................................................3 形態(tài)傷害評價法 ..............................................................................................3 綜合指數(shù)法 ......................................................................................................4 測定鈉離子、氯離子濃度法 ..........................................................................4 大豆種質(zhì)資源耐鹽堿性評價 .......................................................................................53 大豆耐鹽性遺傳、QTL 定位和耐鹽機(jī)理研究進(jìn)展 .........................................................5 經(jīng)典遺傳學(xué)研究 ...........................................................................................................5 單基因控制 .............................................................................................................5 多基因控制 .............................................................................................................6 大豆耐鹽堿 QTL 定位的研究進(jìn)展 .............................................................................6 大豆耐鹽性 QTL 定位研究進(jìn)展 ...........................................................................7 大豆耐堿性 QTL 定位研究進(jìn)展 ..........................................................................8 大豆耐鹽機(jī)理研究進(jìn)展 ...............................................................................................9 鹽脅迫下鹽離子的吸收、外排和轉(zhuǎn)運(yùn) ................................................................9 滲透調(diào)節(jié)機(jī)制 .........................................................................................................9 野生大豆的耐鹽機(jī)理 ..........................................................................................104 關(guān)聯(lián)分析的研究進(jìn)展 .......................................................................................................10 關(guān)聯(lián)分析的原理 .........................................................................................................10 連鎖不平衡 ..........................................................................................................11大豆幼苗期耐鹽堿性的鑒定與關(guān)聯(lián)分析 連鎖不平衡的度量 ..............................................................................................11 影響連鎖不平衡的因素及 LD 衰減 ...................................................................12 關(guān)聯(lián)分析特點 .............................................................................................................13 關(guān)聯(lián)分析的方法、步驟與注意事項 .............................................................................13 關(guān)聯(lián)分析的方法 ...................................................................................................13 關(guān)聯(lián)分析的步驟 ...................................................................................................15 關(guān)聯(lián)分析需注意的問題 .......................................................................................16 關(guān)聯(lián)分析在大豆中的應(yīng)用 .............................................................................................175 本研究的目的與研究內(nèi)容 ...............................................................................................18第二章 試驗材料與分析方法 .............................................................................................211 材料與方法 .......................................................................................................................21 實驗材料 .....................................................................................................................21 大豆幼苗期耐鹽堿相關(guān)性狀考察 .............................................................................21 SSR 全基因組掃描 .....................................................................................................21 實驗儀器 ...............................................................................................................21 DNA 提取 .............................................................................................................21 試劑配制 ............................................................................................................21 DNA 提取步驟 ..................................................................................................22 PCR 擴(kuò)增 ..............................................................................................................22 凝膠電泳檢測 ......................................................................................................23 試劑配制 ............................................................................................................23 凝膠電泳 ...........................................................................................................242 數(shù)據(jù)的分析方法 ...............................................................................................................25 表型數(shù)據(jù)分析 .............................................................................................................25 連鎖不平衡分析 .........................................................................................................25 群體結(jié)構(gòu)分析 .............................................................................................................26 關(guān)聯(lián)分析 .....................................................................................................................26第三章 結(jié)果與分析 .............................................................................................................291 大豆幼苗期耐鹽堿相關(guān)性狀的表型特征、方差及相關(guān)分析 ........................................29 大豆幼苗期耐鹽堿相關(guān)性狀的表型特征分析 ...........................