【正文】 covery was obtained. The particle size does not appear to affect this parameter. In previous studies using a softwood material (pine) at the same pretreatment condition[20], chip size affected strongly the hemicellulosicderived sugar recovery. This could be due, not only to the different hemicellulosicderived sugar position in softwood but different heat transfer into the herbaceous particles. Herbaceous chips may heat more rapidly than wood chips and the size of chips, in the range tested, was not important at temperatures and residence times studied in this work.圖2顯示了復(fù)蘇的hemicellulosicderived(xylosearabinosemannosegalactose)在預(yù)處理后的濾液。Fig2Fig. 3 shows the enzymic hydrolysis yield of the waterinsoluble fibre (expressed as a percentage of the glucose produced in the hydrolysis divided by the potential glucose), after different pretreatments. The enzymic hydrolysis yield depended on the temperature of pretreatment. Enzymic hydrolysis yield increased as the temperature was raised. Lower hydrolysis yields were obtained at 190 8C and 4 and 8 min pretreatment conditions, but there were no significant differences with particle sizes.圖3顯示了酶水解產(chǎn)生的水不溶性纖維(表示為一個(gè)百分比的葡萄糖產(chǎn)生水解除以潛在葡萄糖),在不同的預(yù)處理。這個(gè)事實(shí)并不如此明顯當(dāng)大粒徑(812毫米)都是用在最高溫度測(cè)試(210 8 c)。From the results obtained with B. carinata in this study, we can draw the following conclusions:1) Higher cellulose recoveries at larger particle size (8
12 mm) for all pretreatment conditions tested have been obtained.2) Enzymic hydrolysis yields of 70% were obtained at lower temperature (190 8C) and residence times of 4 and 8 min. Higher enzymic hydrolysis yields (about 99%) were obtained at 210 8C temperature.3) No significant differences in the hemicellulosicsugars recovery were obtained under different pretreatment conditions.4) The best operational conditions were 210 8C, 4
8min and larger particle size, since the highest cellulose recovery and enzymic hydrolysis yields were obtained under such conditions.5) The utilization ofvery small particle size in steamexplosion would not be desirable to optimize the effectiveness of the process and to improve the economy.從得到的結(jié)果carinata b。5)利用ofvery小粒徑在steamexplosion不會(huì)需要優(yōu)化的過程的有效性和改善經(jīng)濟(jì)。carinata稻草的條件化驗(yàn)。13)。這可能是由于,不僅不同hemicellulosicderived糖成分在軟木但不同傳熱到草本粒子。在25毫米粒度纖維素回收率為84%在190 8分鐘,57%在210年c4 8 c8分鐘取得了。 (b) susceptibility of cellulose to enzymic attack。 however, galactose, arabinose and mannose were less vulnerable to acid catalysed degradation and almost quantitative recoveries of sugars solubilised were found in the filtrate.表3顯示了糖成分(g / 100 g的原材料)的濾液預(yù)處理后。carinatastraw。carinata干物質(zhì)由有機(jī)溶劑和水來自采掘,類似于其他草本農(nóng)業(yè)殘留值包括羊茅和柳枝稷[16]。 分析方法原料成分分析以下相應(yīng)的ASTM[1214]。. Enzymic hydrolysisAfter pretreatment the waterinsoluble fibre was enzymically hydrolyzed to determine the maximumobtainable sugar yield. The cellulolytic plex employed in enzymic tests (Celluclast L) was a gift fromNOVO Nordisk (Denmark). Cellulase enzyme loadingwas 15 filter paper unit (FPU)/g substrate. Fungalbglucosidase (Novozyme 188, Novo Ltd.) was used to supplement the bglucosidase activity with an enzyme loading of international unit (IU)/g hydrolysis was performed at 50 8C on a rotary shaker at 150 rpm for 72 h and at 2% (w/v) substrate concentration.。 蒸汽爆破預(yù)處理預(yù)處理的原料是由硬質(zhì)纖維板技術(shù)應(yīng)用。因此,。打開木質(zhì)纖維素的結(jié)構(gòu),廣泛的微生物降解、昂貴的能源需求 ing預(yù)處理過程是必需的。選擇草本作物includingBrassica carinata正在研究潛在的能量來源。Enzymic hydrolysis of steam exploded herbaceous agricultural waste(Brassica carinata) at different particule sizes在不同粒子大小情況下草本農(nóng)業(yè)廢棄物(蕓苔屬植物)的蒸汽爆炸酶水解Abstract摘要The objective of this work was to evaluate the effect of particle size on steamexplosion pretreatment of herbaceous lignocellulosicbiomass. Hemicellulose and cellulose recovery, and effectiveness of enzymic hydrolysis of the cellulosic residue is presented for steamexplosion pretreatment of an agriculture residue (Brassica carinata) using different