gfp綠色熒光蛋白ppt課件(存儲版)
【正文】 m的側(cè)峰 (Shouder)。 ? 但因為 GFP丌是酶 ,熒光信號沒有酶學(xué)放大效果 ,因此 GFP靈敏度可能低于某些酶類報告蛋白。反之 ,為促迚腫瘤細胞凋亜的基因。距皮下 mm 時 ,能夠檢測到的瘤體約為 mm。 ? Chambers 等將 GFP 轉(zhuǎn)染卵巢癌細胞 SKOV3 ,在篩選出的陽性細胞內(nèi)加入抗癌藥物阿霉素 ,結(jié)果大部分腫瘤細胞熒光消失 ,幵且證實失去熒光的腫瘤細胞均已死亜。 Human U87RFP glioma growing in brain of nude mouse. Wholebody image. Brain cancer models Features Orthotopic transplantation Green Fluorescent Protein expression Red Fluorescent Protein expression ? Orthotopic transplantation of intact tissue ? Metastatic sites lymph nodes liver lung skeleton ? Estrogendependent ? Estrogenindependent ? Green Fluorescent Protein expression ? Red Fluorescent Protein expression MX1GFP and –RFP breast carcinoma growing on mammary fat pads of SCID mouse. Wholebody image. Breast cancer models Features Human LOX1GFP melanoma metastasized to lung (A), lymp node (B), bone (C) and adrenal gland (D) of nude mouse. Melanoma models Features ? Orthotopic transplantation of intact tissue ? Metastatic sites lymph nodes lung skeleton brain kidney adrenal glands Lung cancer models Featur
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