蛋白質(zhì)及其標(biāo)記ppt課件-免費閱讀
【正文】 39。15+8 0 o C N NN R 39。 ? 跟熒光蛋白相比，四半胱氨酸標(biāo)簽序列體積小，不會影響被標(biāo)記蛋白質(zhì) 或細(xì)胞的正常生理功能，另外染料量子產(chǎn)率高、穩(wěn)定性強(qiáng)、標(biāo)記速度快 OO O HC O 2 HA s A sS S S SNOO O HA s A sS S S SOH O O HA s A sS S S SOC lC lReAsH (a phenoxaine derivative) CHOxAsH (a dihydroxyxanthone derivative) Tsien et al. Science 1998, 281, 269271. and JACS 2022, 124, 60636076. 雙砷染料 四半胱氨酸體系 雙砷染料 四半胱氨酸重組噬菌體的構(gòu)建及 應(yīng)用 Angew. Chem. Int. Ed., 2022, 50, 58735877 其他特殊標(biāo)記方法： NTA熒光團(tuán)綴合物探針 ?由兩部分組成，一部分為熒光基團(tuán)，另一部分為螯合物， 由金屬離子與含有三醋酸根的叔胺類化合物組成（ NTA） ?六組氨酸 (His6 ) 識別結(jié)合 Ni2+：次氮基三乙酸（ Ni2+:NTA) ? 蛋白質(zhì) C端、 N端、或肽鏈中間 ?比雙砷染料 四半胱氨酸體系通用 The term ‘‘click chemistry’’, originally brought up by K. Barry Sharpless at The Scripps Research Institute in 2022. K. Barry Sharpless and his coworkers have discovered and developed many widely used catalytic oxidation processes, the Sharpless reactions for asymmetric epoxidation, dihydroxylation, and aminohydroxylation of olefins （烯烴的不對稱環(huán)氧化、雙羥化和羥氨化反應(yīng)） . The Nobel Prize, 2022 點擊化學(xué) Azide/Alkyne Cycloaddition L. Pauling. Proc. Natl. Acad. Sci. USA 1933, 19, 860867。用時放至室溫后速取速溶速用。N 3 N NN R 39。 R 39。 Bertozzi, . Science 287, 2022–2022 (2022). Staudinger ligation reaction scheme (azidephosphine conjugation). Phosphineactivated proteins or labeling reagents react with azidelabeled target molecules to form azaylide intermediates that quickly rearrange in aqueous conditions to form stable amide bonds between reactant molecules. Chemoselectivity: phosphines and azides do not occur in cells, they react only with each other in biological samples, resulting in minimal background and few artifacts Compatibility with the design and use of bioorthogonal derivatives. Azide group is extremely small, amino acids, sugars and other building blocks for metabolism can be synthesized to contain azide groups and then supplied to cells as substitutes of their natural counterparts. The selectable azide tag can be incorporated into molecules of interest by the cellular metabolic machinery, allowing the underlying metabolic activity to be detecte
教學(xué)課件相關(guān)推薦
鄂ICP備17016276號-1