聲動(dòng)力治療巨噬細(xì)胞通過促進(jìn)atp釋放趨化單核細(xì)胞科研訓(xùn)練論文-預(yù)覽頁
【正文】 胞對(duì)照組 SDT ATPase組 ATPaseATPase細(xì)胞上清液 ATP酶組SDT組Transwell zVADfmk組 結(jié) 果1. 培養(yǎng)THP1單核細(xì)胞并將其誘導(dǎo)為巨噬細(xì)胞，見圖3 A B圖3 A THP1單核細(xì)胞 B 巨噬細(xì)胞 （200X） 2. Transwell方法測定細(xì)胞上清液對(duì)單核細(xì)胞趨化作用，見圖4 A B C圖4 A Transwell實(shí)驗(yàn)?zāi)Ｊ綀D B 用光學(xué)顯微鏡觀察募集的臺(tái)盼藍(lán)染色后的單核細(xì)胞，并計(jì)數(shù) C 細(xì)胞遷移數(shù) （***：P。BA DC 討論動(dòng)脈粥樣硬化目前已經(jīng)成為了全球范圍內(nèi)，人類健康的嚴(yán)重威脅[20,21]。目前有研究表明，凋亡細(xì)胞釋放的核苷酸可以作為一種“findme”信號(hào)參與對(duì)單核性吞噬細(xì)胞的募集[13]。SDT組較對(duì)照組單核細(xì)胞數(shù)顯著增加。取SDT后20min、對(duì)照組、zVADfmk組及SDTzVAd 組細(xì)胞上清及細(xì)胞裂解液，分別加入配置好的ATP檢測工作液當(dāng)中，反應(yīng)10s后使用luminometer分別檢測細(xì)胞上清液及胞內(nèi)ATP含量。結(jié)論1. 聲動(dòng)力治療巨噬細(xì)胞通過促進(jìn)ATP釋放趨化單核細(xì)胞聚集。特別感謝孫鑫師兄、郭淑媛師姐、陳海波師兄在課題設(shè)計(jì)和實(shí)驗(yàn)過程中給予的悉心指導(dǎo)和無私幫助，在實(shí)驗(yàn)方面的大力支持，在實(shí)驗(yàn)中給予的意見和建議，使我們受到很大的啟發(fā)，為實(shí)驗(yàn)的順利進(jìn)行打下了良好的基礎(chǔ)。衷心地祝愿病理生理教研室的全體老師和同學(xué)工作順利，身體健康！祝愿病理生理教研室永遠(yuǎn)輝煌！最后，感謝哈爾濱醫(yī)科大學(xué)臨床醫(yī)學(xué)七年制的所有同學(xué)在日常學(xué)習(xí)生活中對(duì)我們的幫助和支持！ 參考文獻(xiàn) JJ，F(xiàn)ANG C H．Atheroscleritis is a more rational term for the pathological entity currently known as atherosclerosis[J]．Med Hypotheses，2004，63(1)：100102． JJ．Inflammation：an important mechanism for different clinical entities of coronary artery disease[J]．Chin Med J，2005，118(21)：18171826．3. Rossi ML, Marziliano N, Merlini PA, et al. Different quantitative apoptotic traits in coronary syndromes[J], Circulation, 2004, 110(13):1767773.4. Zorc M, ZorcPleskovic R, VraspirPorenta O, et al. Apoptosis and histopathologic changes in diffuse coronary atherosclerosis [J]. Angiology, 2003, 54(1): 8184.5. Boisvert WA, Rose DM, Boullier A, et al. Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size [J]. Arterioscler Thromb Vasc Biol, 2006, 26(3):563569.6. Schrijvers DM, De Meyer GR, Herman AG, et al. Phagocytosis in atherosclerosis: Molecular mechanisms and implications for plaque progression and stability [J]. Cardiovasc Res, 2007, 73(3): 470480.7. Tabas I. Signal transduction pathways in free cholesterolloaded macrophages: cell biological insight into the progression of atherosclerosis [J]. International Congress Series , 2004, 1 262: 392395.．王雙．動(dòng)脈粥樣硬化中巨噬細(xì)胞凋亡的后果和在治療上的意義：病變階段和吞噬效率的重要性[J]． 中國動(dòng)脈硬化雜志．2005，13(6)：713.9. Trostdorf F, Buchkremer M, Harmjanz A, et al. Fibrous cap thickness and smooth muscle cell apoptosis in highgrade carotid artery stenosis [J]. Eur J Vasc Endovasc Surg, 2005, 29(5):528535.10. Kirti R, Agrawal DK. Cytokins and growth factors invovlesd in apoptosis and proliferation of vascular smooth muscle cells [J]. International Immunoharmacology, 2005, 5(10): 1 487506.11. Salvayre R, Auge N, Benoist H, et al. Oxidized lowdensitu lipoprotein in monocytic cells [J]. Biochim Biophys Acta, 2002, 1 585(23): 213221. S. Ravichandran，F(xiàn)indme and eatme signals in apoptotic cellclearance: progress and conundrums，The Journal of Experimental Medicine,2010,207(9):18071817.13. Michael R. Elliott, Faraaz B. Chekeni, Paul C. Trampont, Eduardo R. Lazarowski, Alexandra Kadl, Scott F. Walk, Daeho Park, Robin I. Woodson, Marina Ostankovich, Poonam Sharma, Jeffrey J. Lysiak,T. Kendall Harden, Norbert Leitinger amp。 Hiroyuki Kusano, MD。 Makoto Kikuchi, PhD。105:149151. VS , Parilak LD, Kerensky RA. Angiographic paterns and the natural history of the vulnerable plaque. Prog Cardiovasc Dis. 2002
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