【正文】 茵、藤黃微球菌以及枯草芽孢桿菌的最小抑制濃度為 mg／ mL，具有良好的開發(fā)牙周保健藥物的潛力。厚樸酚與和厚樸酚對須癬毛癬菌、石膏狀小孢霉、絮狀表皮癬菌、黑曲霉、新生隱球菌、白色念珠菌的最小抑菌濃度（ MIC）均為 5100μg／ mL[23] 。對冠脈結(jié)扎 4 h的大鼠， 10 10 109g／ kg各劑量的厚樸酚能顯著減少心肌梗死范圍 [5]。在超高劑量下， β桉葉油醇和二苯基乙內(nèi)酰脲鈉顯示了更強的阻止電休克癲癇發(fā)作作用， β桉葉油醇有望成為鎮(zhèn)癲劑或二苯基乙內(nèi)酰脲鈉抗癲療法中的協(xié)同治療劑 [4]。厚樸酚在 l100μmol／ L可以增加趨化三肽激活的大鼠中性粒細胞的超氧陰離子，在大于 10 mol／ L時可以明顯地抑制激活的中性粒細胞葡萄糖苷酸酶和溶菌酶的釋放。實驗表明厚樸有明顯抗炎鎮(zhèn)痛作用 [4]。厚樸酚提取物明顯對抗番瀉葉性小鼠腹瀉，對大 鼠膽汁流量有明顯促進作用，表明厚樸與溫里藥一樣具有促進 消化系統(tǒng)的作用。 4． 其他 龍飛首次對厚樸葉化學(xué)成分進行了分離鑒定。此外，還有厚樸木脂素 F等雙木脂素 [15]。干基、干中、干上揮發(fā)油含量依次升高。 圖 厚樸顯微圖 1． 揮發(fā)油 厚樸樹皮揮發(fā)油主要成分為桉葉油醇及其異構(gòu)體，約占揮發(fā)油總量的 40％ 55％，其次是聚傘花素，約占揮發(fā)油總量的 10％ 20％ [2]。無機成分含 鈣、鈉、鉀、鎂、鐵、錳、鋅、銅等 [2]。厚樸的顯微鑒別圖如圖 所示。木栓層為 616 層長方形木栓細胞，細胞壁較薄，木栓化并微木化；木栓形成層區(qū)為 13 列扁平薄壁細胞，栓內(nèi)層由 24 列長方形切向排列的石 細胞組成，石細胞長 4356μm，寬 1632μm，孔溝明顯，壁厚，木化。質(zhì)硬，較易折斷，斷面纖維性。內(nèi)表面紫棕色或深紫褐色，較平滑，具細密縱紋，劃之顯油痕。皮孔為橢圓形皮孔，縱向排列，氣味香，味辛辣，微苦。同時，厚樸是我國貴重的藥用及用材樹種，葉大濃蔭，花大而美麗，為庭園觀賞樹及 行道樹。 5 年生以前生長較慢， 20 年生高達 15 米，胸徑達 20 厘米， 15 年開始結(jié)實， 20 年后進入盛果期。厚樸為喜光的中生性樹種，幼齡期需蔭蔽；喜涼爽、濕潤、多云霧、相對濕度大的氣候環(huán)境。在土層深厚、肥沃、疏松、腐殖質(zhì)豐富、排水良好的微酸性或中性土壤上生長較好，?；焐诼淙~闊葉林內(nèi)，或生于常綠闊葉林緣。 2． 習性 厚樸系溫帶樹種。聚合果長橢圓狀卵圓形 或圓柱狀，長 1012 厘米，直徑 厘米；蓇葖本質(zhì)，頂端有向外彎的椽；種于倒卵圓形，有鮮紅色外種皮。 xxxxxx 大學(xué)本科 畢業(yè)論文 第 IV 頁 Abstract Magnolia is a normal traditional Chinese medicine,， and it has many active effect on abdominal distension and indigestion. Magnolia officlnalis e from dry skin, bark and branch skin of Magnolia officinalis Wils and Magnolia officinalis Rehd． Et wils． var． biloba Rehd． et Wils. Magnolol and honokiol are the main active ingredients, and they have a wide range of pharmacological effects. such as Antiinflammatory, antiarrhythmic, antiepileptic, antiplatelet, antibacterial inhibition and the role of muscle contraction, further more they can inhibit the oxidation products of nitrogen and the expression activity of tumor necrosis factor α. Extract the active ingredients of Chinese medicine in the traditional methods mainly are acid alkaline extraction method, water extraction, ethanol extraction, separation of polyamide extraction, alkaline extraction resin technology, and supercritical CO2 extraction, and Ethanol extraction method in which as a result of cheap, small pollution, and other characteristics suitable for industrial production have been widely used. In this paper, orthogonal experimental design as a tool and extract yield as a quality indicator, we obverse the extraction process of Magnolol and Honokio by extracting in alcohol, focusing on the concentration of ethanol, refluxing time, the number of extraction, solvent usage and other factors on the yield of extract, by their better extraction conditions for extracting time 120min, the concentration of 70% ethanol, 6 times the amount of solvent, extracting the number of 3 times the yield of extract up to %. And with range analysis, variance analysis of experimental data analysis, the relationship between the primary and secondary factors is that the ethanol concentration extract the number of solvent usage extraction time.. Ethanol concentration on the extract yield has the greatest impact, and the yield of extract is highest when Ethanol concentration is 70%. Followed by the extraction of the number, the greater the number of extraction, the higher yield of extract.. Followed by the amount of solvent, approving 6 times the amount of solvent is the best. The impact o f the smallest factor is the extraction time, with the extraction time, higher yield of extract, but after the extraction time 120min, and then to extend the extraction time, but lower yield of extract. So it can be considered to shorten the extraction time, thus reducing energy consumption, enhance economic efficiency. In this paper, the research not only provide a theoretical basis for industrial extraction of the active ingredient Honokiol the design process, but also for indepth study of the pharmacological activity Honokiol provide material basis for the active ingredients of herbs Honokiol extraction and further indepth study guide has a certain significance. key words： Honokiol, extraction process , orthogonal experiment method , ethanol extraction. xxxx 大學(xué)本科 畢業(yè)論文 第 V 頁 目 錄 第 1 章 緒論 …… …………………………………………………………………..………1 厚樸的研究概況 …………………………………………...……..… ..…… ...………1 形態(tài)及習性 …………………………………..……………..……… ...………...…1 性狀鑒 別 及顯微鑒別 ……………………………… ..….. ………… ...…………...2 主要成分 ……………………………….……………………..…………………... 3 藥理作用 …………………………….………………………..…………………...7 臨床研究 ……………………………….……………………..…………………...9 厚樸中厚樸醇與和厚樸酚提取方法 概況 …………..……………..…………….…1 0 堿提酸沉法 …………………………….……………………....……………...…1 1 溶劑提取法 ……… ………………………...……………………...……………..11 聚酰胺分離提取法 ……………………….………………..…………………….1 3 堿提樹脂工藝 …………………………….……..…………………..…………...1 3 超臨界 CO2 萃取 …………………………………...……………………………1 3 本論文研究目的、內(nèi)容及其意義 ……………………………………..……...……13 研究目的 ……………………..……………..……………………………………13 研究內(nèi)容 ………………..………………………………………………..………1 3 研究意義 ………………………..………………………..……………..…..……14 第 2章 厚樸中的厚樸酚 與和厚樸酚提取工藝研究 ………………………...…..…....…15 實驗 原理 …………………..……………………………………………...…………15 實驗儀器、材料與試劑 …………………………………………………...…………15 實驗材料 ………………..…………………………………. ……….……………15 主要試劑 ………………………..….…………………………….………………15 儀器設(shè)備 ……………………..…………………………………..………………15 實驗內(nèi)容 ………………………..…………………………………………………...16 實驗步驟 ………………………..…….………………………………………….16 鑒定 ……………………………..…….………………………………………….17 結(jié)果與討論 ………….……………..……. ………………………………………….1 8 xxxx 大學(xué)本科 畢業(yè)論文 第 VI 頁 實驗 結(jié)果 …………………..………….………………………………………….1 8 結(jié)果 分析 …………………..………….………………………………………….1 8 討論 ………………………..………….………………………………………….2 2 提取過程中出現(xiàn)的問題 …..………….………………………………………….2 3 應(yīng)用前景 ………………..………….…………………………………………….23 結(jié)論 …………………………………… …………………………………………………..2 5 致謝 …………………………………………………………..……………………………………….