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慢病毒顆粒制備和應(yīng)用-全文預(yù)覽

  

【正文】 ates 1 x 107 U/ml 2 x 107 U/ml 3 x 107 U/ml 1 x 107 U/ml ul 2 x 107 U/ml 3 x 107 U/ml ?將慢病毒顆粒稀釋 成不同濃度 (, , and ul)轉(zhuǎn)染細(xì)胞 48~72h后,在熒光顯微鏡下計(jì)數(shù) ?滴度 =感染細(xì)胞數(shù) *病毒顆粒劑量 Titration of the Lentiviruses 1. Florescent colony counting or FACS counting: Infection units (IU/ml) 2. Resistant colony counting: Infection units (IU/ml) 3. RTqPCR: copy number of the virus, not the infection unit Cells for Lentivirus titration : Hela, HT1080, or H1299 cells Purification and Concentration of Lentiviruses 1. Traditional CsCl ultracentrifugation. 3. Ion exchange: ViraBind? Concentration amp。 B: of concentrated lentivirus (incubated 4 hours with LentiDens before centrifuge). C: of concentrated lentivirus (incubated 16 hours with LentiDens before centrifuge). Application of Lentiviruses Products and Services offered by GeneCopoeiaTM 基因表達(dá)克隆 ?預(yù)制的慢病毒表達(dá)克隆 ?基因家族分類(lèi)的克隆 ?按信號(hào)通路分類(lèi)的克隆 ?miRNA表達(dá)克隆 慢病毒轉(zhuǎn)染系統(tǒng) ?慢病毒顆粒 ?慢病毒包裝試劑 ?濃縮純化試劑 ?轉(zhuǎn)染試劑 ?包裝細(xì)胞 表達(dá)譜分析 ?AllinOne? qPCR Array ?AllinOne? miRNA qPCR Array ?AllinOne? qPCR 檢測(cè)試劑盒 ?AllinOne? miRNA qPCR 檢測(cè)試劑盒 ?qPCR array檢測(cè)服務(wù) 。 FastTrap Virus Purification and Concentration Kits (Millipore). 4. Precipitation: LentiPacTM Lentiv
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