【正文】 Martin YR, Robbins TC, Nay LM, Boyd N, Coyne T, Wall MD, Wittwer CT, Teng DH. 2008. Basepair neutral homozygotes can be discriminated by calibrated highresolution melting of small amplicons. Nucleic Acids Res 36:3401–3408.Herrmann MG, Durtschi JD, Bromley LK, Wittwer CT, Voelkerding KV. 2006. Amplicon DNA melting analysis for mutation scanning and genotyping: crossplatform parison of instruments and dyes. Clin Chem 52:494–503.Liew M, Pryor R, Palais R, Meadows C, Erali M, Lyon E, Wittwer C. 2004. Genotyping of singlenucleotide polymorphisms by highresolution melting ofsmall amplicons. Clin Chem 50:1156–1164.McKinney JT, Nay LM, De Koeyer D, Reed GH,Wall M, Palais RA, Jarret RL,Wittwer CT. 2009. Mutation scanning and genotyping in plants by high resolution DNA melting. In: Meksem K, Kahl G, editors. The handbook of plant mutagenesis and mutant screening. Malden: WileyBlackwellVCH, in press.Montgomery J, Wittwer CT, Kent JO, Zhou L. 2007. Scanning the cystic fibrosis transmembrane conductance regulator gene using highresolution DNA melting analysis. Clin Chem 53:1891–1898. NguyenDumont T, Le CalvezKelm F, Forey N, McKayChopin S, Garritano S, GioiaPatricola L, De Silva D, Weigel R, Sangrajrang S, Lesueur F and . Description and validation of highthroughput simultaneous genotyping and mutation scanning by highresolution melting curve analysis. Hum Mutat 30:884–890.Reed GH, Kent JO, Wittwer CT. 2007. Highresolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics 8:597–608.Reed GH, Wittwer CT. 2004. Sensitivity and specificity of singlenucleotide polymorphism scanning by highresolution melting analysis. Clin Chem 50:1748–1754.Seipp MT, Durtschi JD, LiewMA,Williams J, Damjanovich K, PontKingdon G, Lyon E, Voelkerding KV, Wittwer CT. 2007. Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting. J Mol Diagn 9:284–289.Tindall EA, Petersen DC, Woodbridge P, Schipany K, Hayes VM. 2009. Assessing high resolution melt curve analysis for accurate detection of gene variants in plex DNA fragments. Hum Mutat 30:876–883.Vandersteen JG, BayrakToydemir P, Palais RA, Wittwer CT. 2007. Identifying mon genetic variants by highresolution melting. Clin Chem 53:1191–1198.Vossen RHAM, Emmelien A, Roos A, den Dunnen JT. 2009. High Resolution Melting Analysis (HRMA)—more than just sequence variant screening. Hum Mutat 30:860–866.Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP. 1997. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22:130–131, 134–138.Wittwer CT, Rasmussen RP, Ririe KM. 2009. Rapid PCR and melting analysis. In:Bustin SA, editor. The PCR Revolution. Cambridge: Cambridge University Press, in press.Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. 2003. Highresolution genotyping by amplicon melting analysis using LC Green. Clin Chem 49:853–860.Zhou L, Errigo RJ, Lu H, Poritz MA, Seipp MT, Wittwer CT. 2008. Snapback primer genotyping with saturating DNA dye and melting analysis. Clin Chem 54:1648–1656.n HRM在SNP檢測上應(yīng)用van der Stoep N, van Paridon CDM, Janssens T, Krenkova P, Stambergova A, Macek M, Matthijs G, Bakker E. 2009. Diagnostic guidelines for high resolution melting curve analysis: an interlaboratory validation of BRCA1 mutation scanning using the 96well LightScanner (IT). Hum Mutat 30:899–909.Chagn233。54(6):9829.Takano EA, Mitchell G, Fox SB, Dobrovic A. Rapid detection of carriers with BRCA1 and BRCA2 mutations using high resolution melting analysis BMC Cancer. 2008。ger A, Zeinzinger J, Konrad M, Hasenberger P, Allerberger F, Ruppitsch W. Rapid identification of multidrugresistant Mycobacterium tuberculosis isolates by rpoB gene scanning using highresolution melting curve PCR analysis. J Antimicrob Chemother. 2009 Jun。 A, Macek Jr M. Diagnostic