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【正文】 fic PCR (SMARTMSP): highthroughput and probefree quantitative DNA methylation detection. Nucleic Acids Res. 2008。54(12):20558.Steer PA, Kirkpatrick NC, O39。46(3):10907.Chan WF, Maharjan RP, Reeves PR, Sintchenko V, Gilbert GL, Lan R. Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis. J Microbiol Methods. 2009。35(6):e41. Dahl C, Guldberg P. Highresolution melting for accurate assessment of DNA methylation. Clin Chem. 2007。153(Pt 8):267988n 突變Dobrowolski SF, Ellingson CE, Caldovic L, Tuchman M. assessment of gene variants by high resolution melt profiling utilizing the ornithine transcarbamylase gene as a model system. Hum Mutat 28:1133–1140.Dobrowolski SF, Gray J, Miller T, Sears M. 2009. Identifying sequence variants in the human mitochondiral genome using high resolution melt profiling. Hum Mutat 30:891–898.Kramer D, Thunnissen FB, GallegosRuiz MI, Smit EF, Postmus PE, Meijer CJ, Snijders PJ, Heideman DA. A fast, sensitive and accurate high resolution melting (HRM) technologybased assay to screen for mon Kras mutations. Cell Oncol. 2009。130(2):1857.Maltese P, Canestrari E, Palma L, Ruzzo A, Corini F, Menotta M, Andreoni F, Latiano A, Annese V, Magnani M. High resolution melting (HRM) analysis for the detection of ER22/23EK, BclI, and N363S polymorphisms of the glucocorticoid receptor gene. J Steroid Biochem Mol Biol. 2009 Feb。 P, Stambergov225。61(4):48793. Takano T, Ohe Y, Tsuta K, Fukui T, Sakamoto H, Yoshida T, Tateishi U, Nokihara H, Yamamoto N, Sekine I, Kunitoh H, Matsuno Y, Furuta K, Tamura T. Epidermal growth factor receptor mutation detection using highresolution melting analysis predicts outes in patients with advanced non small cell lung cancer treated with gefitinib. Clin Cancer Res. 2007。 36(7):e42.Snell C, Krypuy M, Wong EM。Rourke D, Noormohammadi AH. Classification of fowl adenovirus serotypes by use of highresolution meltingcurve analysis of the hexon gene region. J Clin Microbiol. 2009 Feb。54(2):4326.Lin JH, Tseng CP, Chen YJ, Lin CY, Chang SS, Wu HS, Cheng JC. Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by highresolution melting analysis. J Clin Microbiol. 2008 Mar。4(4). [Epub ahead of print] Wojdacz TK, Dobrovic A. Methylationsensitive high resolution melting (MSHRM): a new approach for sensitive and highthroughput assessment of methylation. Nucleic Acids Res. 2007。374(2):396404.Jeffery N, Gasser RB, Steer PA, Noormohammadi AH. Classification of Mycoplasma synoviae strains using singlestrand conformation polymorphism and highresolution meltingcurve analysis of the vlhA gene singlecopy region. Microbiology. 2007 Aug。8:59.Dufresne SD, Belloni DR, Wells WA, Tsongalis GJ. BRCA1 and BRCA2 mutation screening using SmartCycler II highresolution melt curve analysis. Arch Pathol Lab Med. 2006。 A, Macek Jr M. Diagnostic method validation: High resolution melting (HRM) of small amplicons genotyping for the most mon variants in the MTHFR gene. Clin Biochem. 2009 May 8. [Epub ahead of print]Garritano S, Gemignani F, Voegele C, NguyenDumont T, Le CalvezKelm F, De Silva D, Lesueur F, Landi S, Tavtigian SV. Determining the effectiveness of High Resolution Melting analysis for SNP genotyping and mutation scanning at the TP53 locus. BMC Genet. 2009 Feb 17。13(18 Pt 1):538590.Nomoto K, Tsuta K, Takano T, Fukui T, Fukui T, Yokozawa K, Sakamoto H, Yoshida T, Maeshima AM, Shibata T, Furuta K, Ohe Y, Matsuno Y. Detection of EGFR mutations in archived cytologic specimens of nonsmall cell lung cancer using highresolution melting analysis. Am J Clin Pathol. 2006。 kConFab investigators, Loughrey MB, Dobrovic A. BRCA1 promoter methylation in peripheral blood DNA of mutation negative familial breast cancer patient
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