【正文】 a certain human bone structure, so the use of micro pattern template of electrospun fibers, the posite fiber has a certain structure, more conducive to play the function of . Therefore, the preparation of GE/CS/HA/GO four posite fiber scaffolds for bone repair materials by electrospinning technique and patterned templates. And to explore the four element posite fiber GE/CS/HA/GO electrospinning conditions and the best parameters. The characterization of its morphology and properties. The research content includes the following parts: (1) to explore the optimal concentration of GO, the preparation of four element posite fiber GE/CS/HA/GO under optimal conditions based on GE/CS/HA three system results show that: by light and electron microscopy were obtained when the GO concentration is % and the template for the 200 mesh, the surface of fiber arranged orderly, uniform thickness, no bead and drop. Better morphology. (2) when the materials implanted in the human body, in order to ensure the supply of nutrients required for cells to repair, bone defect, posite materials have a certain adsorption on protein. The adsorption amount of four element posite fiber will be prepared at the optimal conditions of the bovine serum albumin in human body fluid environment. The results show that: the saturated adsorption amount of four yuan of GE/CS/HA/GO posite fiber BSA in %. With the increase of BSA and the pH value of the solution, the adsorption order disordered fiber surface and microstructure of BSA fiber surface is similar, but the effective area of the protein adsorption is different, resulting in the maximum adsorption capacity of BSA on the surface of the material pH value is slightly different. The initial concentration of BSA, the initial adsorption amount of fiber is larger, but it is to achieve balance in 20 min. (3) posite fibrous scaffold after implantation in the human body, such as friction extrusion by the surrounding tissue, an. Therefore, the need to fine fibrous scaffolds with excellent mechanical properties and maximize its function. The four element posite fiber disordering and patterned GE/CS/HA/GO tensile strength test, and pared with the three element posite fiber GE/CS/HA. The results showed that: four yuan three yuan was significantly higher than that of posite fiber IV posite fiber, this is due to the addition of GO in the four element system, improve the quality of fiber bonding interface, which improves the tensile strength of. The four element posite fiber patterning of GE/CS/HA/GO tensile strength reached . Due to the anisotropic microstructure of posite fibers, the tensile strength of the tensile strength of the micro structure fiber membrane is greater than that of disordered fiber. Therefore, the posites exhibit good mechanical strength. The above research results indicate that, this paper successfully prepared GE/CS/HA/GO four element posite fiber, and to characterize the spatial distribution and arrangement, enhance the antibacterial properties and mechanical strength of the posite fiber. Is expected to make the bone repair materials play an important role in the field of clinical medicine. Keywords: graphene oxide。由于微結(jié)構(gòu)復(fù)合纖維的各項(xiàng)異性，微結(jié)構(gòu)纖維膜的拉伸強(qiáng)度大于無序纖維的拉伸強(qiáng)度。因此，優(yōu)良的復(fù)合纖維膜 需要優(yōu)異的力學(xué)性能才能發(fā)揮其最大的功能。mL1 左右。表現(xiàn)出較好的形貌。因此，本文采用靜電紡絲技術(shù)和 圖案化模板 來制備 GE/CS/HA/GO 四元復(fù)合纖維膜 骨修復(fù)材料。這得益于骨修復(fù)材料能直接或間接治療疾病或輔助修復(fù)損傷的骨骼的功能，同時(shí)具有優(yōu)良的生物相容性和可降解性，以及優(yōu)異的機(jī)械強(qiáng)度和抗菌性。這既可以優(yōu)化治療骨病的 方 案 ，降低治療或手術(shù)的風(fēng)險(xiǎn)，而且植入后隨人體代謝而降解，免去二次手術(shù)。并探討 GE/CS/HA/GO 四元復(fù)合纖維的電紡條件和最佳參數(shù) ，并對(duì)其 表征 和性能進(jìn)行 研究 。 （ 2） 當(dāng)材料植入人體內(nèi) ， 為 保證細(xì)胞所需的 營(yíng)養(yǎng)物質(zhì)供給，利于骨缺損處的修復(fù) ，復(fù)合材料需要對(duì)蛋白質(zhì)有一定的吸附 。隨著 BSA 溶液的 pH值的增加，無序纖維表面和微結(jié)構(gòu)纖維表面對(duì) BSA 的吸附順序相似，但由于吸附蛋白的有效面積不同，導(dǎo)致材料表面對(duì) BSA 的最大吸附量的 pH 值略有差別。將無序化和 圖案化 GE/CS/HA/GO 四元復(fù)合纖維 進(jìn)行拉伸強(qiáng)度測(cè)試，并與GE/CS/HA 三元復(fù)合纖維進(jìn)行對(duì)比。因此，該復(fù)合纖維材料表現(xiàn)出 良好的機(jī)械強(qiáng)度。 patterned fiber。其構(gòu)成多種多樣，可以是無機(jī)材料或有機(jī)高分子材料，亦或是復(fù)合材料。可 分為天然生物材料和人造生物材料。 發(fā)展到 90 年代，生物材料技術(shù)發(fā)展迅猛，在當(dāng)時(shí)全球經(jīng)濟(jì) 危機(jī)的大背景下，生物材料卻每年保持著 13%的增長(zhǎng)，這充分體現(xiàn)了生物材料具有廣闊的發(fā)展空間和需求市場(chǎng)。 新疆師范大學(xué) 20xx 屆本科畢業(yè)論文（設(shè)計(jì)） 第 2 頁，共 19 頁 生物材料的分類 生物材料品種多樣，應(yīng)用廣泛，其分類方法 也有多種。 （ 3）耐老化可降解 生物材料在人體內(nèi)有的只有幾小時(shí)或幾天，有的可長(zhǎng)達(dá)幾年甚至十幾年，短時(shí)間內(nèi)其作用完成后應(yīng)在人體內(nèi)自行降解，而長(zhǎng)時(shí)間則需要其具有很強(qiáng)的穩(wěn)定性，能夠在人體內(nèi)長(zhǎng)期發(fā)揮作用。 （ 1）局部組織反應(yīng) ① 排異反應(yīng)：指人體內(nèi)植入異物，會(huì)在其周圍發(fā)生不同程度的炎癥反應(yīng)。例如：材料的性質(zhì)、 材料的機(jī)械運(yùn)動(dòng)、局部營(yíng)養(yǎng)不良、死亡細(xì)胞的沉積等。 ④ 血液反應(yīng)：若生物材料植入人體會(huì)干涉人體循環(huán)系統(tǒng)或與血液接觸就易出現(xiàn)血栓。 （ 2）全身免疫反應(yīng) 生物材料植入人體可能會(huì)引起全身性免疫反應(yīng)，包括體液免疫反應(yīng)和細(xì)胞免疫反應(yīng)。骨修復(fù)支架材料 根據(jù)靶向釋放原理，利用生物材料攜帶促進(jìn)骨生長(zhǎng)的基因定向到達(dá)骨缺損位置，并且緩慢釋放促進(jìn)骨組織的再生，可以提高骨修復(fù)的效果 [1]。明膠的主要成分是多肽分子混合物，分子量可達(dá)幾萬至十幾萬。明膠分子結(jié)構(gòu)上有大量的羥基、羧基和氨基，所以明膠具有極強(qiáng)的親水性。 、印染、新疆師范大學(xué) 20xx 屆本科畢業(yè)論文（設(shè)計(jì)） 第 4 頁，共 19 頁 紡織、國(guó)防、航空、電子等各個(gè)領(lǐng)域發(fā)揮重大作用。殼聚糖具有許多生理活性，如： ， ， ， 排泄重金屬， 。有研究證明羥基磷灰石的晶粒越細(xì)，其生物相容性越高。因此，氧化石墨烯可以降低其存在界面間的能量。 新疆師范大學(xué) 20xx 屆本科畢業(yè)論文（設(shè)計(jì)） 第 5 頁，共 19