離子交換分離原理及設(shè)備-wenkub
2023-02-10 01:20:52 本頁(yè)面
【正文】 SS 柱? 專為 SOURCE 填料設(shè)計(jì)的 FineLINE 柱CHROMAFLOW 自動(dòng)裝卸層析柱? 裝卸柱到清洗都無需打開柱頭? 安全 ,重復(fù)性高? 節(jié)省人力Chromaflow 自動(dòng)裝卸填料生產(chǎn)柱示范錄像STREAMLINE 擴(kuò)張柱床吸附技術(shù)? 配合 STREAMLINE填料和層析柱使用? 粗樣無需離心，過濾，直接上柱? 層析 法回收率高，選擇性多，可自動(dòng)化? 取代離心和超濾設(shè)備216。 色譜分離設(shè)備Column Chromatography實(shí)驗(yàn)室規(guī)模空柱裝柱示范錄像 Gel Filtration size ? Resin has fixed size pores which allow molecules smaller than a given size to enter? Small molecules will take longer to pass down the column than large ones because they travel further? Can estimate molecular weight by parison with a standardIon Exchange charge a)Mixture of proteins is applied to the negatively charged column resin – balanced by Na+ ions (red)b)ve and neutral proteins pass in the void volume, +ve proteins stickc)Excess of Na+ ions is addedd)+ve proteins are elutedCould have used change in pH to elute the proteinsCATION EXCHANGEAffinity Chromatography? Uses specific binding property to
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