響應(yīng)面設(shè)計(jì)優(yōu)化喜樹(shù)葉黃酮提取工藝畢業(yè)論文-wenkub
2023-07-09 22:42:46 本頁(yè)面
【正文】 廣楔形，全緣，邊緣有纖毛，羽脈1011對(duì)；葉柄紅色，有疏毛；花單性同株，白色，無(wú)梗，多數(shù)排成球形頭狀化序，雌花球頂生，雄花球腋生；花期78月，果期1112月，果實(shí)披針形，兩邊有翅；內(nèi)有種子1粒，干縮成細(xì)條狀。【關(guān)鍵詞】響應(yīng)面法； 喜樹(shù)葉； 黃酮提取 IOptimization of Extraction Process of Flavonoids from Camptotheca acuminata leaves Using Response Surface MethodologyAbstractStudies have shown that the content of flavonoids in Camptotheca acuminata leaves is relatively abundant. In order to improve the extraction efficiency of flavonoids in Camptotheca acuminata leaves, studies of the paper used airdry Camptotheca acuminata leaves as raw material, ethanol as extraction agent. In this extraction process, the single factor experiment and response surface experimental design analysis method were applied to reflux extraction, to research reflux extraction conditions and their impact on the yield of flavonoids extraction results, and seek the optimal conditions. The single factor experiments first had five factors that may affect the yield of flavonoids extraction: solidliquid ratio, extraction times, ethanol concentration, extraction temperature and extraction time and the results had shown that the approximate relationship of the various factors and extraction rate, and three significant factors: solidliquid ratio, ethanol volume fraction, extraction temperature. Then the classic three factors and three levels BoxBenhnken experimental design was carried out to establish a quadratic regression model for the extraction efficiency of total flavonoids, and analysis of the experimental data was processed by the DesignExpert software. The optimal conditions of ethanol extract for flavonoids in Camptotheca acuminata leaves were obtained as follows: solidliquid ratio1:40, ethanol volume fraction 60%, extraction temperature 80 176。為提高喜樹(shù)葉中黃酮類化合物的提取效率，本論文在以風(fēng)干喜樹(shù)葉為原料, 用乙醇作提取劑，在喜樹(shù)葉的黃酮提取工藝中, 將單因素試驗(yàn)和響應(yīng)面實(shí)驗(yàn)設(shè)計(jì)分析法應(yīng)用于回流提取喜樹(shù)葉黃酮工藝, 研究回流提取的條件及其對(duì)提取結(jié)果即黃酮的得率的影響，尋求各因素的綜合優(yōu)化條件。本實(shí)驗(yàn)首先對(duì)可能影響喜樹(shù)葉黃酮提取的五個(gè)因素：料液比，提取次數(shù)，乙醇體積分?jǐn)?shù)，提取溫度，每次提取時(shí)間進(jìn)行單因素試驗(yàn)，結(jié)果可以找出各因素與提取率的大致關(guān)系，并選擇了三個(gè)顯著影響因素即料液比，乙醇體積分?jǐn)?shù)，提取溫度，以提取率為響應(yīng)值，進(jìn)行了經(jīng)典的三因素三水平BoxBenhnken 試驗(yàn)設(shè)計(jì)，并通過(guò)DesignExpert軟件對(duì)實(shí)驗(yàn)數(shù)據(jù)進(jìn)行分析，得到相關(guān)響應(yīng)面數(shù)據(jù)和分析結(jié)果。C, extraction time 90min each time and extraction times three times. These conclusions can provide some theoretical reference for improvement of the utilization of Camptotheca flavonoids and their industrial production.[keywords] response surface methodology。喜樹(shù)屬深根性樹(shù)種，性喜溫暖，不耐嚴(yán)寒，適生于年均溫度1317℃的地區(qū)。它們分子中有一個(gè)酮式羰基，第一位上的氧原子具堿性，能與強(qiáng)酸成鹽，其羥基衍生物多具黃色，故又稱黃堿素或黃酮。大量研究顯示，黃酮類化合物有強(qiáng)大的生理活性和藥用價(jià)值，具有抗腫瘤、護(hù)肝、抗炎、抗菌、抗病毒、調(diào)節(jié)內(nèi)分泌系統(tǒng)等諸多功效，同時(shí)還能抑制脂質(zhì)過(guò)氧化作用、血小板凝集、毛細(xì)管滲透性以及脆性等[35]。但目前對(duì)
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