豬i型與ii型干擾素的克隆-生物谷-生物醫(yī)藥門(mén)戶(hù)-wenkub
2023-04-22 03:44:28 本頁(yè)面
【正文】 vities (105 U/mg)Marc145/ PRRSVMarc145/ VSVPK15/ VSVVero/ VSVMDBK/VSVpIFNa246268pIFNgpIFNag1591743 討論 本實(shí)驗(yàn)克隆表達(dá)了豬I型及Ⅱ型干擾素的4個(gè)基因, PoIFNa、PoIFNg、PoIFNag和PoIFN w, 并研究了其抗病毒生物學(xué)活性。在體外實(shí)驗(yàn)中, 用細(xì)胞病變抑制法比較了原核表達(dá)的4種豬干擾素的抗病毒活性。 表達(dá)和復(fù)性工藝未能使干擾素恢復(fù)成具有生物活性的蛋白質(zhì)。REFERENCES[1] Lefevre F, Boulay V. A novel and atypical type one interferon gene expressed by trophoblast during early pregnancy. J Biol Chem, 1993, 268: 1976019768.[2] Roberts RM, Liu L, Alexenko A. New and atypical families of type I interferons in mammals: Comparative functions, structures, and evolutionary relationships. Prog Nucl Acid Res Mol Biol, 1997, 56: 287325.[3] Pestka S, Krause CD, Walter MR. Interferons, interferonlike cytokines, and their receptors. Immunol Rev, 2004, 202: 832.[4] Chevaliez S, Pawlotsky JM. Interferons and their use in persistent viral infections. Handb Exp Pharmacol, 2009, 189: 20341.[5] Hauptmann R, Swetly P. A novel class of human type I interferons. Nucl Acids Res, 1985, 13(13): 47394749.[6] Hughes AL. The evolution of the type I interferon gene family in mammals. J Mol Evol, 1995, 41: 539548.[7] Yang LM, Xue QH, Liu WJ, et al. Cloning and characterization of a novel feline IFNomega. J Interferon Cytokine Res, 2007, 27(2): 11927.[8] Himmler A, Hauptmann R, Adolf GR, at al. Structure and expression in Escherichia coli of canine interferonalpha genes. J Interferon Res, 1987, 7(2): 17383.[9] Mege D, Lefevre F, Labonnardiere C. The porcine family of interferonomega: Cloning, structural analysis, and functional studies of five related genes. J Interferon Res, 1991, 11(6): 34150.[10] Overend C, Mitchell R, He D, et al. Rebinant swine beta interferon protects swine alveolar macrophages and MARC145 cells from infection with porcine reproductive and respiratory syndrome virus. J Gener Virol, 2007, 88: 925931.[11] Moraes MP, de Los Santos T, Koster M, et al. Enhanced antiviral activity against footandmouth disease virus by a bination of type I and II porcine interferons. J Virol, 2007, 81(13): 712435.[12] Zhang LX, Zhang TF, Wang JZ. The Protein Protocols Handbook. Beijing: Science Press, 2000: 4246. 張龍翔, 張庭芳, 汪家政. 蛋白質(zhì)手冊(cè). 北京: 科學(xué)出版社, 2000: 4246. [13] John A, Armstrong. Semimicro dyebinding assay for rabbit interferon. Appl Microbiol, 1971, 21(4): 723725.[14] Sara Rubinstein, Philip C, Familletti, et al. Convenient assay for interferons. J Virol, 1981, 37(2): 755758.[15] Chinese Pharmacopoeia Commission. The Pharmacopoeia of the People’s Republic of China. Beijing: Chemical Industry Press, 2005, appendix XC: 5657. 國(guó)家藥典委員會(huì)編. 中華人民共和國(guó)藥典. 北京: 化學(xué)工業(yè)出版社, 2005, 附錄X C: 5657.[16] Lefevre F, La Bonnardiere C. Molecular cloning and sequencing of a gene encoding biologically active porcine alphainterferon. J Interferon Res, 1986, 6(4): 34960.[17] Cao RB, Xu XQ, Zhou B, et al. Gene modi
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