【正文】 Watanabe, M. F.。 Fujiki, H., Inbitro and invivo effects of protein phosphatase inhibitors, microcystins and nodularin, on mouse skin and fibroblasts. Biochem. Biophys. Res. Commun. 1990, 171 (2),867874. 7. Honkanan, R. E.。 Bruno, M.。 Lagana, A., Mornitoring algal toxins in lake water by liquid chromatography tandem mass spectrometry. Environ. Sci. Technol. 20xx, 40, 29172923. 9. Harada, .。 Watanabe, M. F.。 Carmichael, W. W., Analysis and purification of toxic peptides from cyanobacteria by reversedphase highperformance liquid chromatography. Journal of Chromatography A 1988, 448, 275283. 10. Meriluoto, J.。 5～ 14 周：實驗室實驗。 并以水華藍(lán)藻銅綠微囊藻為研究對象，通過四環(huán)素對銅綠微囊藻的生長抑制、 四環(huán)素 對藻液pH 值的影響、 四環(huán)素 對蛋白質(zhì)含量的影響，研究四環(huán)素對藍(lán)藻的急性毒性。 關(guān)鍵詞： 四環(huán)素、銅綠微囊藻、急性毒性、抑制、降解 Acute toxicity of tetracycline on Microcystis aeruginosa Abstract: Tetracycline antibiotics are widely used in the world. This is one of the largest types of antibiotics, which can remain in the environment and bring potential environmental risks. In this study, the ecotoxicity of tetracyclines on cyanobacteria Microcystis aeruginosa was studied. Growth inhibition of this kind of algae exposure to tetracycline was measured. pH values, protein content, were also studied to determine the acute toxicity of tetracycline on M. aeruginosa. The results showed that at low concentration 1 mg/L tetracycline can stimulate the growth of M. aeruginosa, and in prolonged exposure to high concentrations of tetracycline, M. aeruginosa growth was suppressed. This paper also detected the degradation of tetracycline in M. aeruginosa culture medium by high performance liquid chromatography (HPLC). The results showed that the reaction is a firstorder kiics reaction, Ln (tetracycline concentration) and the reaction time showed linear relationship. And discussed the future research directions, aimed to provide valuable reference for the ecological risk assessment of the antibiotics. KeyWords: Tetracycline。 Degradation 目錄 1. 引言（緒論） .......................................................................................................................... 1 抗生素的污染現(xiàn)狀 ......................................................................................................... 1 四環(huán)素類抗生素的污染現(xiàn)狀 ......................................................................................... 2 四環(huán)素類抗生素的生態(tài)毒性 ......................................................................................... 3 四環(huán)素類抗生素對微生物的生態(tài)毒性 ............................................................... 3 四環(huán)素類抗生素對植物的生態(tài)毒性 ................................................................... 4 四環(huán)素類抗生素對動物的生態(tài)毒性 ................................................................... 4 四環(huán)素的 理化性質(zhì)和藥理毒理 ..................................................................................... 5 四環(huán)素的理化性質(zhì) ............................................................................................... 5 四環(huán)素的藥理毒理 ............................................................................................... 5 藍(lán)藻、銅綠微囊藻 ......................................................................................................... 6 藍(lán)藻概述 ............................................................................................................... 6 銅綠微囊藻概述 ................................................................................................... 6 藻類水華 ............................................................................................................... 6 微囊藻毒素的污染現(xiàn)狀 ................................................................................................. 7 微囊藻毒素的污染現(xiàn)狀 ....................................................................................... 7 天然及水庫水體的微囊藻毒素污染 ................................................................... 7 飲用 水的微囊藻毒素污染 ................................................................................... 8 課題研究的意義和目的 ................................................................................................. 8 課題研究的內(nèi)容 ............................................................................................................. 8 2. 實驗條件及預(yù)實驗 .................................................................................................................. 9 實驗條件 ......................................................................................................................... 9 實驗儀器 ............................................................................................................... 9 實驗材料 ............................................................................................................... 9 實驗藥品 ............................................................................................................. 11 預(yù)實驗 ........................................................................................................................... 11 BG11 培養(yǎng)液的配置 ............................................................................................ 11 高壓蒸氣滅菌 ..................................................................................................... 12 銅綠微囊藻的 接種 ............................................................................................. 12 銅綠微囊藻的 培養(yǎng) ............................................................................................. 12 實驗方案論證 ............................................................................................................... 12 3. 實驗方案與方法 .................................................................................................................... 14 四環(huán)素儲備液的配置 ................................................................................................... 14 銅綠微囊藻細(xì)胞數(shù)與吸光度的關(guān)系 ........................................................................... 14 實驗原理 .............................