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2期 王賀正等:水稻開花期一些生理生化特性與品種抗旱性的關(guān)系 1水稻開花期一些生理生化特性與品種抗旱性的關(guān)系王賀正,馬 均,李旭毅,李 艷,張榮萍,汪仁全(四川農(nóng)業(yè)大學(xué)水稻研究所,溫江 611130)摘要:【目的】探索水稻開花期適應(yīng)干旱環(huán)境的生理生化機(jī)制,篩選水稻開花期抗旱性鑒定的生理生化指標(biāo)?!痉椒ā坷每购敌圆煌?0個(gè)水、旱稻品種,在干旱棚內(nèi)通過開花期水分脅迫,研究了葉片抗壞血酸(Vc)、游離氨基酸、可溶性蛋白質(zhì)、丙二醛(MDA)等物質(zhì)的含量和保護(hù)性酶活性的變化?!窘Y(jié)果】開花期水分脅迫增加了葉片Vc、游離氨基酸、可溶性蛋白質(zhì)和MDA含量,提高了超氧化物歧化酶(SOD)、過氧化氫酶(CAT)和過氧化物酶(POD)的活性。相關(guān)分析表明, 在水分脅迫與對(duì)照條件下Vc、游離氨基酸、MDA含量和SOD活性的相對(duì)值與品種的抗旱系數(shù)有顯著或極顯著相關(guān)性?!窘Y(jié)論】水、旱條件下Vc、游離氨基酸總量、MDA含量和SOD活性相對(duì)值可作為水稻開花期抗旱性鑒定指標(biāo),利用此4項(xiàng)相對(duì)值與抗旱系數(shù)所建立的回歸方程可預(yù)測品種開花期的抗旱性。關(guān)鍵詞:水稻;水分脅迫;開花期;生理生化性狀Relationship Between Some Physiological and Biochemical Characteristics and Drought Tolerance at Rice Flowering Stage WANG Hezheng, MA Jun, LI Xuyi, LI Yan, ZHANG Rongping, WANG Renquan(Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130) Abstract: 【Objective】Rice may suffer serious water stress duringtheir growth period,which affects the process of physiological metabolic activitys. So scientists studied physiological and biochemical characteristics of drought tolerance in rice..【Method】Ten rice cultivars with different drought tolerances were used as experimental materials. Changes in ascorbic acid (Vc) content, free amino acid content, soluble protein content, malondialdehyde (MDA) content and antioxidative enzyme activities were studied under both irrigation and drought conditions at the flowering stage. 【Result】 The results indicated that water stress increased contents of Vc , free amino acid , soluble protein and malondialdehyde (MDA), as well as the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in leaves at the flowering stage. Correlation analysis showed that the drought tolerant coefficient significantly or very significantly correlated with relative contents of Vc , free amino acid , MDA and SOD activity under both irrigation and drought conditions. 【Conclusion】Therefore, they could be selected as identification indexes for drought tolerance at flowering stage. Using stepwise regression equation to evaluate the drought tolerance of rice cultivars was considered scientifically reliable. Key words: Rice。 Water stress。 Flowering stage。 Physiological and biochemical characteristics0 引言【研究意義】水稻是中國主要糧食作物之一,其用水量占農(nóng)業(yè)用水總量的65%以上[1]。隨著中國缺水問題的日趨嚴(yán)重,水稻旱作、旱種呈現(xiàn)不斷擴(kuò)大趨勢,但是,旱作水稻品種在抗旱性、產(chǎn)量和品質(zhì)上受到一定限制。然而,植物在長期進(jìn)化過程中,為保護(hù)自身免受傷害會(huì)產(chǎn)生一系列適應(yīng)性反應(yīng)。因