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【正文】 （二號(hào)黑體空行）固始雞免疫器官內(nèi)細(xì)胞凋亡基因Fas和FasL的動(dòng)態(tài)表達(dá)（論文題目二號(hào)黑體居中）（二號(hào)黑體空行）梅承君（四號(hào)仿宋居中）（2007屆動(dòng)物科學(xué)專業(yè)1班）（五號(hào)楷體居中）（五號(hào)楷體空行）摘要（小五黑體）：為探討（小五楷體。論文英文、英文縮寫(xiě)和阿拉伯?dāng)?shù)字均用Times New Roman格式）固始雞免疫器官內(nèi)細(xì)胞凋亡基因Fas和FasL的動(dòng)態(tài)表達(dá)（目的），應(yīng)用免疫組織化學(xué)技術(shù)和Leica 顯微圖像處理系統(tǒng)，對(duì)細(xì)胞凋亡基因Fas和FasL在固始雞免疫器官內(nèi)的動(dòng)態(tài)表達(dá)進(jìn)行了研究（方法）。結(jié)果：Fas在不同發(fā)育階段固始雞免疫器官內(nèi)均有表達(dá)，但表達(dá)量均不相同，呈波浪樣動(dòng)態(tài)變化；Fas表達(dá)于固始雞免疫器官內(nèi)淋巴細(xì)胞細(xì)胞膜和細(xì)胞質(zhì)，不表達(dá)于細(xì)胞核；Fas表達(dá)陽(yáng)性細(xì)胞在固始雞不同免疫器官內(nèi)分布位置不同，呈散在或簇團(tuán)狀分布：法氏囊內(nèi)陽(yáng)性細(xì)胞主要分布于黏膜靠近上皮細(xì)胞的固有膜層內(nèi)、淋巴小結(jié)與淋巴小結(jié)之間區(qū)域、淋巴小結(jié)邊緣，淋巴小結(jié)內(nèi)少量淋巴細(xì)胞也有Fas表達(dá)；胸腺內(nèi)主要分布于胸腺小葉髓質(zhì)，極少出現(xiàn)在皮質(zhì)內(nèi)；脾臟內(nèi)主要分布于紅髓和邊緣區(qū)、淋巴小結(jié)周圍和動(dòng)脈周圍淋巴鞘周圍的區(qū)域，動(dòng)脈周圍淋巴鞘極少有Fas表達(dá)，淋巴小結(jié)無(wú)Fas表達(dá)。FasL在固始雞免疫器官內(nèi)的表達(dá)與Fas相似，但表達(dá)量與Fas相比較少（結(jié)果）。以上結(jié)果表明，細(xì)胞凋亡基因Fas和FasL參與了固始雞免疫器官內(nèi)淋巴細(xì)胞發(fā)育分化過(guò)程中的凋亡調(diào)控，并對(duì)免疫器官的穩(wěn)定發(fā)揮重要作用（結(jié)論）。關(guān)鍵詞（小五黑體）：細(xì)胞凋亡；Fas；FasL；凋亡基因表達(dá)；免疫組織化學(xué)；免疫器官；固始雞（小五號(hào)楷體）（五號(hào)楷體空行）Dynamic Expression of Apoptosis Gene Fas and FasL in Immunity Organs of Gushi Chickens（三號(hào)Times New Roman居中，實(shí)詞首字母均應(yīng)該大寫(xiě)）（五號(hào)楷體空行）MEI Chengjun（四號(hào)Times New Roman居中）(Class 1 of Animal Science, Graduated in 2007)（小四Times New Roman居中，實(shí)詞首字母均應(yīng)該大寫(xiě)）（小四楷體空行）Abstract（小五Times New Roman加黑）: Dynamic(小五Times New Roman) expression of apoptosis gene Fas and FasL in immunity organs of Gushi chickens(Objective) was studied by using the immunohistochemical techniques and Leica imageprocessing system(Method). The results showed that Fas gene expressed in the immunity organs at different developmental stages, but the expression products varied and the dynamic changes were wave. Fas expressed in the cell membrane and the cytoplasm in the immunity organs, while negatively in the nucleus of lymphocytes. Fas gene positively expressed cells distributed in the various location in the different immunity organs, scatteredly or in clusters. The positively expressed cells in the bursa were mainly found in the lamina propria of mucous membrane, among the lymphoid nodules and at the edges of lymphoid nodules. Fas also expressed in a few lymphocytes inside the lymphoid nodules. The positive cells in the thymus localized mainly in the thymic medulla, and a few in the thymic cortex. Fas predominantly expressed in the red pulp, the marginal zones, and the zones around the splenic nodules and the periarterial lymphatic sheath in the spleen. The expression of Fas gene was faintly positive in the periarterial lymphatic sheath and negative in the splenic nodules. The expression of FasL in the immunity organs of Gushi chickens was similar to that of Fas, but the expression product of FasL was less than that of Fas(Results). All results mentioned above indicated that Fas and FasL genes participated in the regulation of the lymphocytes apoptosis in the immunity organs of Gushi chickens, and played the important roles in keeping the immunity organs stable(Conclusion). （英文摘要盡量用過(guò)去時(shí)和被動(dòng)語(yǔ)態(tài)；堅(jiān)決不能用在線翻譯）Key words（小五Times New Roman加黑）: Apoptosis。 Fas。 FasL。 Expression of apoptosis gene。 Immunohistochemistry。 Immunity organ。 Gushi chicken（關(guān)鍵詞要用實(shí)詞，小五Tim
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