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牧醫(yī)工程學院本科學生論文電子模版doc(存儲版)

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【正文】 n of 4week Gushi chicken: The positive Fascells, in spleen, distributed in red pulp scatteredly or in clusters. The positive Fasprotein was yellowish brown, and found in cell membrane and cytoplasm of lymphocytes. (400)6. Expression of Fas in thymus of 12week Gushi chicken. The positive Fascells, in thymus, were localized scatteredly in thymic medulla. (200)7. Expression of FasL in Bursa of 12week Gushi chicken: The positive FasLcells were scattered, or in clusters, and observed in lamina propria of mucous membrane between epithelium and lymphoid nodules, a few in edges of lymphoid nodules.(200)8. Expression of FasL in Bursa of 4week Gushi chicken: The positive FasLcells were scattered, or in clusters, and distributed in lamina propria of mucous membrane between epithelium and lymphoid nodules. (200)縮略詞Ca:中央動脈。(200)2.4周齡固始雞法氏囊內凋亡基因Fas的表達:Fas表達陽性細胞分布于法氏囊淋巴小結邊緣和內部、淋巴小結與淋巴小結之間區(qū)域,散在或簇團狀分布。本試驗是在河南省家禽種質資源創(chuàng)新工程研究中心進行的,在此期間田亞東、孫桂榮等老師給予了大力幫助和支持,在此表示由衷的感謝!(五號宋體空行)參考文獻:(小五黑體)[1] 袁紅, 楊明清. Fas/FasL與凋亡研究進展[J]. 國外醫(yī)學-生理、病理科學與臨床分冊, 2000, 20(1): 26~28.(小五宋體或Times New Roman。 關于FasL在固始雞免疫器官內的表達特點及其生理意義FasL在固始雞法氏囊內的表達部位與Fas相似,但表達量較少。另外基因突變可導致Fas和FasL的表達發(fā)生障礙[28]。正常生理情況下淋巴細胞的凋亡,對于促進B淋巴細胞的增值分化和成熟具有重要的生理意義[1]。 BC注:同列字母不同表示差異顯著(P)。 BC177。 C177。9 / 9由表1可知,F(xiàn)as在不同發(fā)育階段固始雞脾臟內均有表達,但表達量有所不同。陽性細胞分布于上皮層與淋巴小結之間靠近上皮層的固有膜區(qū)域、淋巴小結邊緣、淋巴小結與淋巴小結之間固有膜區(qū)域,淋巴小結內部少量淋巴細胞也有Fas表達。2 結果與分析免疫組織化學結果的判定參照標準(陽性細胞判定標準):經免疫組織化學染色的切片,背景無色或淺藍色。顯微鏡:Leica DM 2000;攝像頭:CH9435 Heerbrugg (DFC 320);軟件處理系統(tǒng):Leica QWin 軟件。研究不同發(fā)育階段固始雞免疫器官淋巴細胞凋亡機制,對研究免疫器官的穩(wěn)定、環(huán)境與動物有機體,特別是與免疫的關系[21~25],調節(jié)免疫器官發(fā)育,制定合理的免疫程序等都具有重要意義。Fas系統(tǒng)功能正常對機體自身穩(wěn)定具有重要作用,F(xiàn)as功能過強或缺陷均可導致疾病發(fā)生[2]。 Immunity organ。結果:Fas在不同發(fā)育階段固始雞免疫器官內均有表達,但表達量均不相同,呈波浪樣動態(tài)變化;Fas表達于固始雞免疫器官內淋巴細胞細胞膜和細胞質,不表達于細胞核;Fas表達陽性細胞在固始雞不同免疫器官內分布位置不同,呈散在或簇團狀分布:法氏囊內陽性細胞主要分布于黏膜靠近上皮細胞的固有膜層內、淋巴小結與淋巴小結之間區(qū)域、淋巴小結邊緣,淋巴小結內少量淋巴細胞也有Fas表達;胸腺內主要分布于胸腺小葉髓質,極少出現(xiàn)在皮質內;脾臟內主要分布于紅髓和邊緣區(qū)、淋巴小結周圍和動脈周圍淋巴鞘周圍的區(qū)域,動脈周圍淋巴鞘極少有Fas表達,淋巴小結無Fas表達。關鍵詞(小五黑體):細胞凋亡;Fas;FasL;凋亡基因表達;免疫組織化學;免疫器官;固始雞(小五號楷體)(五號楷體空行)Dynamic Expression of Apoptosis Gene Fas and FasL in Immunity Organs of Gushi Chickens(三號Times New Roman居中,實詞首字母均應該大寫)(五號楷體空行)MEI Chengjun(四號Times New Roman居中)(Class 1 of Animal Science, Graduated in 2007)(小四Times New Roman居中,實詞首字母均應該大寫)(小四楷體空行)Abstract(小五Times New Roman加黑): Dynamic(小五Times New Roman) expression of apoptosis gene Fas and FasL in immunity organs of Gushi chickens(Objective) was studied by using the immunohistochemical techniques and Leica imageprocessing system(Method). The results showed that Fas gene expressed in the immunity organs at different developmental stages, but the expression products varied and the dynamic changes were wave. Fas expressed in the cell membrane and the cytoplasm in the immunity organs, while negatively in the nucleus of lymphocytes. Fas gene positively expressed cells dist
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