蛋白質生化分離技術(已修改)
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【正文】 Bioseparation of proteins Chapter 1 Introduction ? 1 Introduction ? Biomaterial solution properties: ? (1) Dilute solution ? (2) Components plicate ? (3) Unstable ? (4) Batches difference ? Bioseparation requirements ? (1) High purity ? (2) Large scale ? (3) Economical ? (4) Biopatible New words and vocabulary ?1 cell fusion ?2 interest protein ?3 biopatible ?4 purification ?5 biological material ?6 plant cell culture ?7 fermentation ?8 strategies II The Need for bioseparation ? Process requirements ? (1) Safety ? (2) Quality ? (3) Amount ? (4) Preventing Posttranslational change ? (5) Low cost ? What will bioseparation do? ? (1) Modification of existing procedures ? (2) Developing new and novel bioseparation technology ? Difficulty of generalized algorithm processes and subtle difference of different proteins New words and vocabulary ? Protocol (方案） ? Algorithm ( 法則） ? Generalized (通用的，一般化的） ? Invaluable (有價值的） ? Inactivate (失活的） ? Therapeutic (治療的 ) ? Viable (可行的） III Classification of bioseparation steps(1) ? Bioseparation procedures ?(1) Removal of insolubles ?(2) Isolation of product ?(3) Purification ?(4) Polishing III Classification of bioseparation steps (2) ? Bioseparation unit operation ? (1) Cell disruption ? (2) Centrifugation ? (3) Chromatography ? (4) Drying ? (5) Evaporation ? (6) Extraction ? (7) Filtration ? (8) Membrane separation ? (9) Precipitation III Classification of bioseparation steps (3) ?Quality requirements: ? Products free from: ? (1) detergent ? (2) endotoxins ? (3) proteases ? (4) immunogenic substance ? (5) microbiological contamination ? (6) modified proteins ? (7) nucleid acids ? (8) oligonucleotide ? (9) enterotoxins III Classification of bioseparation steps (4) ? Therapeutic usage requirements: ? (1) Potency ? (2) Identity ? (3) Abnormal toxicity ? (4) Nucleic acids ? (5) Homogeneity ? (6) Absence of conflicting activity ? Process requirements: ? (1) Maximize product yield in initial step ? (2) Limit the number of steps ? (3) Intensification II
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