【正文】 榆林學(xué)院本科畢業(yè)論文 I 分類號(hào) 單位代碼 11395 密 級(jí) 學(xué) 號(hào) 1006220219 學(xué)生畢業(yè) 論文 題 目 響應(yīng)曲面法優(yōu)化陜北豆腐渣 中水不溶性膳食纖維提取工藝的研究 作 者 *** 院 (系 ) 化學(xué)與化工學(xué)院 專 業(yè) 化學(xué) 指導(dǎo)教師 *** 答辯日期 *年 * 月 * 日 響應(yīng)曲面法優(yōu)化陜北豆腐渣中水不溶性膳食纖維提取工藝的研究 II 摘 要 膳 食纖維是功能性食品的重要基料。豆腐渣中因含有豐富的膳食纖維成分 成為 最近食品界研究的熱點(diǎn)。 本論文 通過(guò)對(duì)堿濃度，酶濃度，酶解溫度，酶解時(shí)間進(jìn)行單因素實(shí)驗(yàn)， 以水不溶性膳食纖維的純度為考察指標(biāo)，確定出提取水不溶性膳食纖維的最佳條件。在單因素試驗(yàn)基礎(chǔ)上， 根據(jù) BoxBehnken 中心組合設(shè)計(jì)原理 ，選取酶濃度、酶解溫度和酶解時(shí)間 3 因素 進(jìn)行響應(yīng)曲面分析，建立了豆腐渣中水不溶性膳食纖維提取率的二次多項(xiàng)數(shù)學(xué)模型。實(shí)驗(yàn)結(jié)果表明豆腐渣中水不溶性膳食纖維最佳提取工藝條件為： 酶濃度 4%，酶解溫度 60oC，酶解時(shí)間 5h,在此條件下水不溶性膳食纖維提取率為 %。 關(guān)鍵詞： 豆腐渣；水不溶性膳食纖維； 響應(yīng)曲面；提取工藝 榆林學(xué)院本科畢業(yè) 論文 III Study the Method of Response Surface Optimized Extraction Technology for Insoluble Dietary Fiber of Bean Curd Residue in Shanbei ABSTRACT Dietary fiber is an important basic material of functional food. Bean curd residue because of containing rich dietary fiber bees the hot spot in food research recently. In this paper, the highest purity based on the single factor experiment of alkali concentration, enzyme concentration, reaction temperature and hydrolysis time, determined the optimal extraction of IDF. According to the design principle of BoxBehnken center bination, set up the quadratic mathematical models of insoluble dietary fiber extraction rate in bean curd residue by the singlefactor, selected enzyme concentration, reaction temperature and hydrolysis time 3 factors response surface analysis. The results showed that the optimal extraction conditions were enzyme concentration of 4%, reaction temperature of 60oC and hydrolysis time of 5 h. In conditions of the optimal extraction, the extraction rate of IDF was %. Keywords: Bean curd residue。 insoluble dietary fiber。 response surface。 extraction technology 響應(yīng)曲面法優(yōu)化陜北豆腐渣中水不溶性膳食纖維提取工藝的研究 IV 目 錄 摘 要 ................................................................................................................................ II ABSTRACT ....................................................................................................................... III 目 錄 ............................................................................................................................... IV 1 緒 論 ................................................................................................................................ 1 膳食纖維的定義及組成 ............................................................................................ 1 膳食纖維的定義 ............................................................................................ 1 膳食纖維的組成 ............................................................................................ 2 水溶性膳食纖維和水不溶性膳食纖維 ....................................................................... 2 膳食纖維的生理功能 ............................................................................................... 3 預(yù)防肥胖癥的 功效 ........................................................................................ 3 預(yù)防心血管疾病的功效 ................................................................................. 3 降低血壓的功效 ............................................................................................ 3 預(yù)防糖尿病的功效 ........................................................................................ 3 緩解便秘，預(yù)防腸道疾病的功效 ................................................................... 4 制取水不溶性膳食纖維的原料 — 豆腐渣 ................................................................... 4 豆腐渣膳食纖維的研究現(xiàn)狀 ..................................................................................... 4 從陜北豆腐渣中制取水不溶性膳食纖維的目的和意義 .............................................. 5 用響應(yīng)曲面法優(yōu)化陜北豆腐渣中水不溶性膳食纖維提取工藝的意義 ......................... 5 2 實(shí)驗(yàn)部分 ........................................................................................................................... 7 實(shí)驗(yàn)材料、儀器及試劑 ........................................................................................... 7 實(shí)驗(yàn)材料 ...................................................................................................... 7 實(shí)驗(yàn)儀器 ...................................................................................................... 7 實(shí)驗(yàn)試劑 ..................................................................................................... 7 陜北豆腐渣中提取水不溶性膳食纖維工藝流程 ........................................................ 8 陜北豆腐渣中提取水不溶性膳食纖維工藝的操作步驟 .............................................. 8 沖洗烘干 ...................................................................................................... 8 粉碎過(guò)篩 ...................................................................................................... 8 稱取樣品 ...................................................................................................... 8 加純水 .......................................................................................................... 8 堿處理 .......................................................................................................... 8 調(diào) PH ........................................................................................................... 8 榆林學(xué)院本科畢業(yè) 論文 V 酶解 ............................................................................................................. 8 滅酶 ............................................................................................................. 9 抽濾 ............................................................................................................. 9 烘干 ............................................................................................................ 9 稱量 ....................................................................................................