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rotocol for sterilization of BioFlow 110 3 Liter Bioreactor. Document : VP001 Revison: Effective Date: January 1, 2020 Page 2 of 6 3. References: Guide to Operations: BioFlow 110 Modular Benchtop Fermentor Manual M1273 0054 Revision E Guide to Operations: AMSCO Eagle Series Autoclave Model 3011 Revision SOP P001 Cleaning of NBS Bioflow 110 BioFlow 110 Modular Benchtop Fermentor 3 Liter vessel. SOP P004 –Preparation of NEB BioFlow 110 Modular Benchtop Fermentor 3 Liter vessel. SOP P005 – Collection of Inprocess samples from NEB BioFlow 110 Modular Benchtop Fermentor 3 Liter vessel. SOP Q001 – Determination of the number of viable bacterial cells through serial dilution and growth on LuriaBertani media. SOP Q002 Gram Staining. SOP P006 – Preparation of Luria Bertani liquid media. SOP P008 – Determination of the Optical Density of a bacterial culture 4. Reagents: 70% Isopropyl alcohol Sample collection vials LB liquid media Bacillus subtilus culture Culture tubes, inoculating loops, bunsen burners. _____________________________________End of Page 2 _______________________________ The Northeast Biomanufacturing Center amp。 Collaborative Validation PlanFermentation Vessel Sterilization Subject: Validation Protocol for sterilization of BioFlow 110 3 Liter Bioreactor. Document : VP001 Revison: Effective Date: January 1, 2020 Page 3 of 6 5. Responsibility: It is the responsibility of all personnel validating the performance of the equipment covered by this validation plan to read and understand the validation plan. It is the responsibility of the Quality Control Laboratory to perform the viable cell counts on pre and post autoclaved material described in this validation plan. It is the responsibility of the validation department to