【正文】
Clean environment ③ Young and vigorous Explant selection Explant selection 1. Select a suitable potato mother plants (high productivity, high quality, free from diseases and so on…) the selected mother plants under more hygienic conditions 熱處理脫毒 ? 脫毒:室內(nèi)發(fā)芽,熱處理 (38℃ ) 2 周; 原理: 當(dāng)植物組織處于高于正常溫度的環(huán)境( 3540℃ )時(shí),組織內(nèi)部的病毒部分或全部 鈍化 ,但寄主植物的組織很少或不會(huì)受到傷害。 Treatment of virus free Excise a shoot tips or buds from potato tubes after treatment of high temperature (38℃ , 2 weeks) for virusfree 莖尖培養(yǎng)脫毒 原理 ? 病毒的分布不均勻 , 越靠近莖頂?shù)母腥驹捷p , 生長點(diǎn) ( 約 - ) 幾乎不含或含病毒很少 。 原因是 無維管束 , 病毒只能通過 胞間連絲傳遞 , 不及細(xì)胞不斷分裂和生長的速度 。因此可利用微莖尖培養(yǎng)脫毒種苗 。 ? 。 Initiation of culture 1 stage Purpose Establishment of aseptic cultures in vitro Operation Take explants from mother plants such as buds leaf pieces, flowers… into the vessels. Sterile Explant Put on medium Wash with steriled water Take bud under aseptic conditions ? Establishment of aseptic cultures in vitro ? ? Medium ? ? Culture environment cond